您好,欢迎访问中国水产科学研究院 机构知识库!

Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis

文献类型: 外文期刊

作者: Sha, Z-X. 1 ; Luo, X-H. 1 ; Liao, X-L. 1 ; Wang, S-L. 2 ; Wang, Q-L. 1 ; Chen, S-L. 1 ;

作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China

2.Auburn Univ, Fish Mol Genet & Biotechnol Lab, Dept Fisheries & Allied Aquacultures, Auburn, AL 36849 USA

3.Auburn Univ, Program Cell & Mol Biosci, Aquat Genom Unit, Auburn, AL 36849 USA

关键词: cross-species amplification;Cynoglossus semilaevis;EST-SSR;polymorphism

期刊名称:JOURNAL OF FISH BIOLOGY ( 影响因子:2.051; 五年影响因子:2.25 )

ISSN: 0022-1112

年卷期: 2011 年 78 卷 1 期

页码:

收录情况: SCI

摘要: Sixty novel simple sequence repeat (SSR) markers were developed from expressed sequence tags (EST) of half-smooth tongue sole Cynoglossus semilaevis exploited in the laboratory. The number of alleles, observed and expected heterozygosity per locus ranged from two to 16, from 0.0833 to 1.0000 and from 0.0816 to 0.913, respectively. Of these SSRs, 20 had significant homology to known genes by BLASTx (basic local alignment search tool x) search. For cross-species amplification, there are 53 positive amplifications in Japanese flounder Paralichthys olivaceus with 12 polymorphic loci and 51 positive amplifications in Senegalese sole Solea senegalensis with 11 polymorphic loci. These new EST-SSR markers will be useful for genetic studies and genome mapping of C. semilaevis and its closely related fishes. (C) 2010 The Authors

  • 相关文献

[1]MHC polymorphism and disease resistance to vibrio anguillarum in 8 families of half-smooth tongue sole (Cynoglossus semilaevis). Du, Min,Chen, Song-lin,Liu, Yang,Yang, Jing-feng,Du, Min,Du, Min,Liu, Yan-hong. 2011

[2]Cloning of Disease Resistance-relevant Nramp and MHC IIB Genes and their Application Potential in Molecular Breeding for Disease Resistance in Japanese Flounder (Paralichthys olivaceus). S.L. Chen,Y.X. Zhang,Z.J. Wang,Z.X. Sha,M.Y. Xu,Chinese Academy of Fishery Sciences; Chinese Academy of Sciences;. 2005

[3]Development of New Microsatellite DNA Markers from Apostichopus japonicus and Their Cross-Species Application in Parastichopus parvimensis and Pathallus mollis. Liao, Meijie,Wang, Yingeng,Rong, Xiaojun,Zhang, Zheng,Li, Bin,Wang, Lan,Chen, Guiping. 2011

[4]Isolation of novel microsatellite markers from Paralichthys lethostigma (Paralichthyidae) and their cross-species application in Pleuronectiformes. Xu, Y. -J.,Liao, M. -J.,Wang, Y. -G.,Liu, Z. -C.,Qin, P.,Zhang, Z.,Rong, X. -J.,Liu, X. -Z.. 2013

[5]Isolation and characterization of 31 polymorphic microsatellite markers in barfin flounder (Verasper moseri) and the cross-species amplification in spotted halibut (Verasper variegatus). Ma, Hongyu,Chen, Songlin,Ma, Hongyu. 2009

[6]Polymorphic microsatellite markers in the false kelpfish Sebastiscus marmoratus: isolation, characterization, and cross-species amplification. Liu, H. B.,Liu, H. B.,Liu, S. F.,Ye, J. B.,Yuan, Y. J.,Zhuang, Z. M.,Yuan, Y. J.,Ding, S. X.. 2014

[7]Development of thirty-four novel polymorphic microsatellite markers in Coilia ectenes (Clupeiformes: Engraulidae) and cross-species amplification in two closely related taxa. Chen, Fang,Zeng, Liyan,Cheng, Qiqun,Chen, Fang. 2012

[8]Development of 20 Microsatellite Markers for Solenocera crassicornis and Their Cross-Species Application in Solenocera melantho. Liu, Shufang,Liu, Hongbo,Yuan, Yanjiao,Dai, Fangqun,Zhuang, Zhimeng,Liu, Hongbo,Lin, Lin,Yuan, Yanjiao. 2012

[9]Isolation and characterization of polymorphic microsatellite loci from an EST-library of red sea bream (Chrysophrys major) and cross-species amplification. Chen, SL,Liu, YG,Xu, MY,Li, J. 2005

[10]Development of thirty-five novel polymorphic microsatellite markers in Pseudosciaena polyactis (Perciformes:Sciaenidae) and cross-species amplification in closely related species, Pseudosciaena crocea. Chen, Wenming,Cheng, Qiqun,Chen, Wenming.

[11]Isolation and characterization of polymorphic microsatellite loci from a repeat-enriched genomic library of stone flounder (Kareius bicoloratus) and cross-species amplification. Tian, Yong-Sheng,Miao, Gui-Dong,Shao, Chang-Wei,Liao, Xiao-Lin,Chen, Song-Lin.

[12]Development of 12 polymorphic microsatellite markers in Coilia ectenes Jordan and Seale, 1905 (Clupeiformes: Engraulidae) and cross-species amplification in Coilia mystus Linnaeus, 1758. Ma, Chunyan,Cheng, Qiqun,Zhang, Qingyi.

[13]Isolation via enrichment and characterization of 14 dinucleotide microsatellite loci in one catfish, Glyptosternum maculatum and cross-amplification in other related taxa. Guo, Baoying,Xie, Congxin,Li, Hongjing,Shan, Lei,Wang, Huanling,Huo, Bin,Zhou, Qing,Zhang, Yan.

[14]Isolation and characterization of polymorphic microsatellite loci from a dinucleotide-enriched genomic library of starry flounder (Platichthys stellatus) and cross-species amplification. Miao, Guidong,Ma, Hongyu,Liao, Xiaolin,Chen, Songlin,Miao, Guidong,Shao, Changwei,Ma, Hongyu.

[15]Identification of type I microsatellite markers associated with genes and ESTs in Scylla paramamosain. Ma, Hongyu,Ma, Chunyan,Ma, Lingbo.

[16]Transcriptome analysis between invasive Pomacea canaliculata and indigenous Cipangopaludina cahayensis reveals genomic divergence and diagnostic microsatellite/SSR markers. Mu, Xidong,Song, Hongmei,Luo, Du,Gu, Dangen,Xu, Meng,Luo, Jianren,Hu, Yinchan,Hou, Guangyuan,Xu, Peng,Zhang, Jiaen. 2015

[17]Constructing a genetic linkage map and mapping quantitative trait loci for skeletal traits in Japanese flounder. Liu, Yi,Zhang, Xiaoyan,Si, Fei,Sun, Zhaohui,Wang, Guixing,Wang, Yufen,Liu, Yi,Liu, Yongxin,Liu, Yingjie,Yang, Runqing,Liu, Haijin.

[18]基于公共数据库牙鲆EST-SSR的开发及特征分析. 刘奕,陈伟兴,周丹,陈晓婷,王美玉,王桂兴,刘海金. 2013

[19]牙鲆EST资源的SSR信息分析. 陈松波,龚丽,刘海金. 2010

[20]利用EST-SSR座位对鲤鱼几种生长性状的单标记回归分析. 徐美佳,张研,鲁翠云,张晓峰,刘继红,孙效文. 2009

作者其他论文 更多>>