Dumbbell-like upconversion nanoparticles synthesized by controlled epitaxial growth for light-heat-color tri-modal sensing of carcinoembryonic antigen
文献类型： 外文期刊
作者： Shao, Kang 1 ; Xie, Wuyan 1 ; Ling, Qingqing 1 ; Wang, Tao 1 ; Zhang, Hongxi 1 ; Teng, Yuanjie 1 ; Ye, Shiyi 2 ; Yuan, Xiufang 2 ; Pan, Zaifa 1 ;
作者机构： 1.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310002, Peoples R China
3.Zhejiang Res Inst Chem Ind, Hangzhou 310023, Zhejiang, Peoples R China
关键词： Tri-modal sensing; Upconversion nanoparticles; Photothermal; Colorimetric detection; Carcinoembryonic antigen
期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.6； 五年影响因子：10.8 ）
ISSN： 0956-5663
年卷期： 2023 年 228 卷
页码：
收录情况： SCI
摘要： Accurate quantitative analysis of tumor markers in a wide linear range has important practical significance towards complex clinical samples in cancer identification and monitoring of tumor development stages, but remains challenging. Herein, three-layer dumbbell-like upconversion nanoparticles NaErF4:Tm@NaYF4@-NaNdF4 (labeled as UCNPs) combined with G-quadruplex (G4) DNAzyme are reported for tri-modal sensing of carcinoembryonic antigen (CEA) in a wide range using upconversion luminescence (UCL), photothermal and catalysis signal readouts. Initially, dumbbell-like UCNPs were controlled synthesized by a three-dimensional epitaxial growth strategy through tuning the concentration of Nd precursors. After surface functionalization, G4zyme-UCNPs-cDNA/Apt-MB was subsequently fabricated by biotin-streptavidin interaction and DNA hybrid-ization. Quantitative detection of CEA was achieved by competitive interaction and magnetic separation, and the intensities of tri-modal signals (light, heat and catalysis-based chrominance) of dissociative probes are linearly related to the concentration of CEA. The results showed that the tri-modal sensing method exhibited a wide linear range (0.005-2000 ng/mL) and low limit of detection (LOD) across three models: the luminescence model (0.005-50 ng/mL, LOD = 0.910 pg/mL), the catalysis model (10-1000 ng/mL, LOD = 0.387 ng/mL), and the temperature model (50-2000 ng/mL, LOD = 1.114 ng/mL). These findings suggest that the tri-modal sensing platform is suitable for use in the analysis of a wide range of complex and diverse clinical samples.
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