您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Dumbbell-like upconversion nanoparticles synthesized by controlled epitaxial growth for light-heat-color tri-modal sensing of carcinoembryonic antigen

文献类型： 外文期刊

作者： Shao, Kang 1 ; Xie, Wuyan 1 ; Ling, Qingqing 1 ; Wang, Tao 1 ; Zhang, Hongxi 1 ; Teng, Yuanjie 1 ; Ye, Shiyi 2 ; Yuan, Xiufang 2 ; Pan, Zaifa 1 ;

作者机构： 1.Zhejiang Univ Technol, Coll Chem Engn, Hangzhou 310014, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310002, Peoples R China

3.Zhejiang Res Inst Chem Ind, Hangzhou 310023, Zhejiang, Peoples R China

关键词： Tri-modal sensing; Upconversion nanoparticles; Photothermal; Colorimetric detection; Carcinoembryonic antigen

期刊名称：BIOSENSORS & BIOELECTRONICS （ 影响因子：12.6； 五年影响因子：10.8 ）

ISSN： 0956-5663

年卷期： 2023 年 228 卷

页码：

收录情况： SCI

摘要： Accurate quantitative analysis of tumor markers in a wide linear range has important practical significance towards complex clinical samples in cancer identification and monitoring of tumor development stages, but remains challenging. Herein, three-layer dumbbell-like upconversion nanoparticles NaErF4:Tm@NaYF4@-NaNdF4 (labeled as UCNPs) combined with G-quadruplex (G4) DNAzyme are reported for tri-modal sensing of carcinoembryonic antigen (CEA) in a wide range using upconversion luminescence (UCL), photothermal and catalysis signal readouts. Initially, dumbbell-like UCNPs were controlled synthesized by a three-dimensional epitaxial growth strategy through tuning the concentration of Nd precursors. After surface functionalization, G4zyme-UCNPs-cDNA/Apt-MB was subsequently fabricated by biotin-streptavidin interaction and DNA hybrid-ization. Quantitative detection of CEA was achieved by competitive interaction and magnetic separation, and the intensities of tri-modal signals (light, heat and catalysis-based chrominance) of dissociative probes are linearly related to the concentration of CEA. The results showed that the tri-modal sensing method exhibited a wide linear range (0.005-2000 ng/mL) and low limit of detection (LOD) across three models: the luminescence model (0.005-50 ng/mL, LOD = 0.910 pg/mL), the catalysis model (10-1000 ng/mL, LOD = 0.387 ng/mL), and the temperature model (50-2000 ng/mL, LOD = 1.114 ng/mL). These findings suggest that the tri-modal sensing platform is suitable for use in the analysis of a wide range of complex and diverse clinical samples.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台