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CRISPR-Cas13a-Based Detection for Bovine Viral Diarrhea Virus

文献类型： 外文期刊

作者： Yao, Rui 1 ; Xu, Yueren 1 ; Wang, Lang 1 ; Wang, Dawei 1 ; Ren, Linchang 1 ; Ren, Changling 1 ; Li, Cunyuan 2 ; Li, Xiaoy 1 ;

作者机构： 1.Shihezi Univ, Coll Life Sci, Shihezi, Peoples R China

2.Shihezi Univ, Coll Anim Sci & Technol, Shihezi, Peoples R China

3.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China

关键词： CRISPR-Cas13a; BVDV; detection; virus; LwCas13a

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）

ISSN：

年卷期： 2021 年 8 卷

页码：

收录情况： SCI

摘要： Bovine Viral Diarrhea Virus (BVDV) is the main pathogen of bovine viral diarrhea disease (BVD), which leads to enormous economic losses in the cattle industry. A sensitive and specific detection for BVDV is advantageous to the control of BVDV. Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have been used for detecting virus RNA. In this study, the expression and purification of LwCas13a protein was optimized and the RNase activity of LwCas13a in vitro was verified. CRISPR-LwCas13a system could detect BVDV virus and BVDV RNA with high specificity and simplicity. The detection limit of the LwCas13a system was 10(3) pM, and there were no cross-reactions with HEK293T and MDBK. In summary, a sensitive, specific, and simple nucleic acid detection method based on CRISPR-Cas13a was developed for BVDV. This method provides a new detection strategy for early diagnosis of BVDV.

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