16S rRNA gene sequencing analysis on changes in the intestinal flora of Procambarus clarkii with "Black May" disease
文献类型： 外文期刊
作者： Gong, Jie 1 ; Shen, Guoqing 1 ; Zhu, Mengru 1 ; Zhan, Ming 1 ; Xi, Changjun 1 ; Shui, Yan 2 ; Xu, Zenghong 2 ; Shen, Huaishun 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Nanjing 210095, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Jiangsu, Peoples R China
关键词： Procambarus clarkii; "Black May" disease (BMD); intestinal flora; high-throughput sequencing
期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.554； 五年影响因子：1.448 ）
ISSN： 2096-5508
年卷期：
页码：
收录情况： SCI
摘要： The morbidity and mortality peak of farmed Procambarus clarkii occurs around May every year, a phenomenon known as "Black May" disease (BMD). Increasing evidence shows that the intestinal flora is closely related to host health. We analyzed and compared the microbiota of healthy and BMD-affected P. clarkii intestines. The results show that there was no significant difference in bacterial alpha-diversity (richness P=0.59; evenness P=0.43; and diversity P=0.052) between the diseased group and the control group. Four dominant phyla in the intestines of crayfish in the control group, namely Tenericutes (30.86%), Bacteroidetes (29.99%), Firmicutes (22.23%), and Proteobacteria (15.23%), were identified. However, a striking shift in the microbial composition were found in the intestines of P. clarkii with BMD. Bacteroidetes was a dominant phylum in healthy P. clarkii, whereas the prevalence was low in diseased P. clarkii (1.87%). By contrast, the prevalence of Proteobacteria was significantly higher (P<0.05) in P. clarkii with BMD than in P. clarkii without BMD. Candidatus Bacilloplasma, Bacteroides, Vibrio, and Aeromonas showed significant differences (P<0.05) at the genus level. Tax4Fun function prediction indicated that the relative abundance of genes involved in energy metabolism in the intestinal flora of P. clarkii with BMD was significantly reduced (P<0.05). Therefore, BMD can change the composition of the intestinal microbiota of P. clarkii. This study contributes to the understanding of the relationship between intestinal flora and host especially in aquatic animals.
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