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RNA-seq and phytohormone analysis reveals the culm color variation of Bambusa oldhamii Munro

文献类型： 外文期刊

作者： Jiao, Yulian 1 ; Zeng, Hu 3 ; Xia, Haitao 1 ; Wang, Yueying 1 ; Wang, Jinwang 1 ; Jin, Chuan 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, Wenzhou, Zhejiang, Peoples R China

2.Zhejiang Prov Collaborat Innovat Ctr Bamboo Resou, Linan, Zhejiang, Peoples R China

3.Wuhan Metware Biotechnol Co Ltd, Wuhan, Peoples R China

关键词： Bambusa oldhamii; Culm color variation; Phytohormone; Transcription factors; MYB; HY5; ABA; GA(1); GA(7); RNA-seq

期刊名称：PEERJ （ 影响因子：3.061； 五年影响因子：3.537 ）

ISSN： 2167-8359

年卷期： 2022 年 10 卷

页码：

收录情况： SCI

摘要： Background. The clumping bamboo Bambusa oldhamii Munro, known as "green bamboo", is famous for its edible bamboo shoots and fast-growing timber. The green and yellow striped-culm B. oldhamii variety, named B. oldhamii f. revoluta W.T. Lin & J. Y. Lin, is an attractive system for researching the culm color variation of B. oldhamii. Methods. Millions of clean reads were generated and assembled into 604,900 transcripts, and 383,278 unigenes were acquired with RNA-seq technology. The quantification of ABA, IAA, JA, GA(1), GA(3), GA(4), and GA(7) was performed using HPLC-MS/MS platforms. Results. Differential expression analysis showed that 449 unigenes were differentially expressed genes (DEGs), among which 190 DEGs were downregulated and 259 DEGs were upregulated in B. oldhamii f. revoluta. Phytohormone contents, especially GA(1) and GA(7), were higher in B. oldhamii. Approximately 21 transcription factors (TFs) were differentially expressed between the two groups: the bZIP, MYB, and NF-YA transcription factor families had the most DEGs, indicating that those TFs play important roles in B. oldhamii culm color variation. RNA-seq data were confirmed by quantitative RT-PCR analysis of the selected genes; moreover, phytohormone contents, especially those of ABA, GA(1) and GA(7), were differentially accumulated between the groups. Our study provides a basal gene expression and phytohormone analysis of B. oldhamii culm color variation, which could provide a solid fundamental theory for investigating bamboo culm color variation.

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