中国水产科学研究院机构知识库

Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA

收藏
分享
全文链接

文献类型：外文期刊

作者： Ren, Weicheng ¹ ; Renault, Tristan ² ; Cai, Yuyong ¹ ; Wang, Chongming ¹ ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

2.Ifremer, Lab Genet & Pathol, F-17390 La Tremblade, France

3.Ocean Univ China, Dept Aquaculture, Qingdao 266003, Shandong, Peoples R China

关键词： Ostreid herpesvirus 1 (OsHV-1);Oyster;Loop-mediated isothermal amplification (LAMP);Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （影响因子：2.014；五年影响因子：2.001 ）

ISSN： 0166-0934

年卷期： 2010 年 170 卷 1-2 期

页码：

收录情况： SCI

摘要： A loop-mediated isothermal amplification (LAMP) assay was developed for rapid, specific and sensitive detection of ostreid herpesvirus 1 (OsHV-1) DNA. A set of four primers was designed, based on the sequence of the ATPase subunit of the OsHV-1 DNA-packaging terminase gene. The reaction temperature and time were optimized to 64 degrees C and 60 min, respectively. LAMP products were detected by agarose gel electrophoresis or by visual inspection of a color change due to addition of fluorescent dye. The developed method was highly specific for detection of OsHV-1, and no cross-reaction was observed with other DNA viruses, such as White spot syndrome virus (WSSV), Penaeus stylirostris densovirus (PstDNV). Turbot reddish body iridovirus (TRBIV) and Lymphocystis disease virus (LCDV) found commonly in China. The lower detection limit of the LAMP assay was approximately 20 copies per reaction, and it was 100 times more sensitive than that of conventional PCR. A comparative evaluation of 10 oyster samples using LAMP and PCR assays showed overall correlation in positive and negative results for OsHV-1. These results indicate that the LAMP assay is a simple, rapid, sensitive, specific and reliable technique for the detection of OsHV-1. The LAMP technique has capacity for use for the detection of OsHV-1 both in the laboratory and on farms. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.

相关文献

[1]Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification. Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo,Zhang Fengying,Shi Yanhong,Jiang Keji,Xu Zhaoli,Ma Lingbo. 2012

[2]An efficient method of noroviruses recovery from oysters and clams. Zhou Deqing,Ma Liping,Zhao Feng,Yao Lin,Li Xinguang,Ma Liping,Su Laijin,Li Xinguang. 2013

[3]Characterization of microbial community in high-pressure treated oysters by high-throughput sequencing technology. Cao Rong,Zhao Ling,Sun Huihui,Liu Qi. 2018

[4]OYSTER AND ASSOCIATED BENTHIC MACROFAUNAL DEVELOPMENT ON A CREATED INTERTIDAL OYSTER (CRASSOSTREA ARIAKENSIS) REEF IN THE YANGTZE RIVER ESTUARY, CHINA. Quan, Weimin,Shen, Xinqiang,Chen, Yaqu,Humphries, Austin T.. 2012

[5]Development of A Monoclonal Antibody-Coated Immunomagnetic Beads for Separation and Detection of Norovirus (Genogroup II) in Oysters. Yao, Lin,Jiang, Yanhua,Jiang, Wei,Li, Fengling,Zhai, Yuxiu,Wang, Lianzhu. 2013

[6]Biotransformation and detoxification of inorganic arsenic in Bombay oyster Saccostrea cucullata. Zhang, Wei,Guo, Zhiqiang,Zhou, Yanyan,Zhang, Li,Zhou, Yanyan,Liu, Huaxue.

[7]Application of an improved loop-mediated isothermal amplification detection of Vibrio parahaemolyticus from various seafood samples. Li, Chunhou,Xu, Zhenbo,Wang, Li,Li, Yue,Zhong, Qingping,Zhao, Xihong,Xu, Zhenbo,Chu, Jin,Li, Yanyan. 2011

[8]Detection of LAMP Products Using CNT Array Electrode. Zhang, Xuzhi,Li, Qiufen,Chen, Bijiuan,Ma, Shaosai,Cui, Yi. 2011

[9]Development of a loop-mediated isothermal amplification assay for rapid detection of iridovirus in the Chinese giant salamander. Meng, Yan,Zhang, Hui,Liang, Hongwei,Zeng, Lingbing,Xiao, Hanbing,Meng, Yan,Xie, Congxin. 2013

[10]Sensitive and rapid detection of Karenia mikimotoi (Dinophyceae) by loop-mediated isothermal amplification. Zhang, Fengying,Ma, Lingbo,Xu, Zhaoli,Zheng, Junbin,Shi, Yanhong,Lu, Yanan,Miao, Yuping.

[11]Establishment and application of cross-priming isothermal amplification coupled with lateral flow dipstick (CPA-LFD) for rapid and specific detection of red-spotted grouper nervous necrosis virus. Su, Zi Dan,Shi, Cheng Yin,Huang, Jie,Shen, Gui Ming,Li, Jin,Wang, Sheng Qiang,Fan, Chao,Shi, Cheng Yin,Huang, Jie,Su, Zi Dan,Shen, Gui Ming,Wang, Sheng Qiang,Fan, Chao. 2015

[12]Detection and quantification of hepatopancreatic parvovirus in penaeid shrimp by real-time PCR assay. Liu, Tianqi,Yang, Bing,Song, Xiaoling,Wang, Xiuhua,Yuan, Yanyan,Liu, Li,Huang, Jie,Liu, Tianqi,Yuan, Yanyan. 2013

[13]An improved method for detection of Edwardsiella tarda by loop-mediated isothermal amplification by targeting the EsrB gene. Xie Guosi,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Huang Jie,Xie Guosi,Huang Jie. 2012

[14]Development and validation of a TaqMan (TM) fluorescent quantitative real-time PCR assay for the rapid detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Xie Guosi,Huang Jie. 2012

[15]Development of cross-priming amplification coupled with vertical flow visualization for rapid detection of infectious spleen and kidney necrosis virus (ISKNV) in mandarin fish, Siniperca chuatsi. Liu, Wenzhi,Zhou, Yong,Fan, Yuding,Jiang, Nan,Zeng, Lingbing,Cain, Kenneth,Cain, Kenneth. 2018

[16]A real-time PCR targeted to the upstream regions of HlyB for specific detection of Edwardsiella tarda. Xie Guosi,Huang Jie,Zhang Qingli,Han Nana,Shi Chengyin,Wang Xiuhua,Liu Qinghui,Xie Guosi,Huang Jie. 2012

[17]Rapid diagnosis of turbot reddish body iridovirus in turbot using the loop-mediated isothermal amplification method. Zhang, Qingli,Shi, Chenyin,Huang, Jie,Jia, Kuntong,Chen, Xinhua,Liu, Hong. 2009

[18]Hazard and Detection of Vibrio parahaemolyticus in Seafood. Wang, Yuan,Shen, Xiaoshen,Huang, Xuanyun,Cai, Youqiong. 2011

[19]Development of a reverse transcription loop-mediated isothermal amplification assay for rapid detection of grass carp reovirus. Zhang, Qing-Li,Yan, Yi,Shi, Cheng-Yin,Wang, Qin-Tao,Huang, Jie,Shen, Jin-Yu,Hao, Gui-Jie,Liu, Hong. 2013

作者其他论文更多>>

A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor

作者：Huang, Bowen;Bai, Changming;Li, Chen;Wang, Chongming;Xin, Lusheng;Liu, Qin;Xin, Lusheng

关键词：insulin-like growth factor type 1 receptor; ferritin; iron metabolism; fibronectin type III domain; Scapharca broughtonii
Iron Regulatory Protein 1 Inhibits Ferritin Translation Responding to OsHV-1 Infection in Ark Clams, Scapharca Broughtonii

作者：Huang, Bowen;Zhang, Xiang;Bai, Changming;Li, Chen;Wang, Chongming;Xin, Lusheng;Huang, Bowen;Zhang, Xiang;Bai, Changming;Li, Chen;Wang, Chongming;Xin, Lusheng;Liu, Qin

关键词：iron regulatory protein 1; ferritin; iron metabolism; Scapharca broughtonii; OsHV-1
Complete Genome Sequence of Vibrio kanaloae Strain KH-4 From Ark Clams, Scapharca broughtonii

作者：Xin, Lusheng;Yu, Jiangnan;Huang, Bowen;Li, Chen;Bai, Changming;Wang, Chongming;Xin, Lusheng;Yu, Jiangnan;Xin, Lusheng;Yu, Jiangnan;Li, Chen;Bai, Changming;Wang, Chongming;Liu, Qin

关键词：ark clam; Vibrio; Vibrio kanaloae; genome; comparative
Characterization of Host Cell Potential Proteins Interacting with OsHV-1 Membrane Proteins

作者：Yu, Jiangnan;Huang, Bowen;Li, Chen;Wang, Dandan;Yao, Mengli;Xin, Lusheng;Bai, Changming;Wang, Chongming;Yu, Jiangnan;Huang, Bowen;Li, Chen;Wang, Dandan;Yao, Mengli;Xin, Lusheng;Bai, Changming;Wang, Chongming;Yu, Jiangnan;Liu, Ying

关键词：ostreid herpesvirus 1; membrane proteins; interaction; hemocytes; pull-down
Isolation and Characterization of Vibrio kanaloae as a Major Pathogen Associated with Mass Mortalities of Ark Clam, Scapharca broughtonii, in Cold Season

作者：Huang, Bowen;Li, Chenghua;Huang, Bowen;Zhang, Xiang;Wang, Chongming;Bai, Changming;Li, Chen;Xin, Lusheng

关键词：Vibrio kanaloae; Scapharca broughtonii; low temperature; hemolytic activity; siderophore production
Influence of temperature on the pathogenicity of Ostreid herpesvirus-1 in ark clam, Scapharca broughtonii

作者：Xin, Lusheng;Wei, Zhixin;Bai, Changming;Huang, Bowen;Wang, Chongming;Xin, Lusheng;Wei, Zhixin;Bai, Changming;Huang, Bowen;Wang, Chongming;Chen, Hao

关键词：Ostreid herpesvirus 1; Temperature; Mortality; OsHV-1 DNA load; Scapharca broughtonii
Isolation and characterization of Vibrio harveyi as a major pathogen associated with mass mortalities of ark clam, Scapharca broughtonii, in summer

作者：Wei, Zhixin;Zhang, Weiwei;Li, Chenghua;Wei, Zhixin;Xin, Lusheng;Bai, Changming;Wang, Chongming;Li, Chenghua

关键词：Scapharca broughtonii; Vibrio harveyi; Pathogen identification; Temperature regulation

Development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of ostreid herpesvirus 1 DNA

作者其他论文 更多>>

A Putative Receptor for Ferritin in Mollusks: Characterization of the Insulin-like Growth Factor Type 1 Receptor

Iron Regulatory Protein 1 Inhibits Ferritin Translation Responding to OsHV-1 Infection in Ark Clams, Scapharca Broughtonii

Complete Genome Sequence of Vibrio kanaloae Strain KH-4 From Ark Clams, Scapharca broughtonii

Characterization of Host Cell Potential Proteins Interacting with OsHV-1 Membrane Proteins

Isolation and Characterization of Vibrio kanaloae as a Major Pathogen Associated with Mass Mortalities of Ark Clam, Scapharca broughtonii, in Cold Season

Influence of temperature on the pathogenicity of Ostreid herpesvirus-1 in ark clam, Scapharca broughtonii

Isolation and characterization of Vibrio harveyi as a major pathogen associated with mass mortalities of ark clam, Scapharca broughtonii, in summer

意 见 箱

作者其他论文更多>>

意见箱