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QTL-seq and marker development for resistance to head splitting in cabbage

文献类型： 外文期刊

作者： Zhu, Xiaowei 1 ; Tai, Xiang 1 ; Ren, Yunying 1 ; Wang, Min 1 ; Chen, Jinxiu 1 ; Bo, Tianyue 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Hort Res, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China

关键词： Cabbage; Head splitting; Resistance; QTL-seq; Candidate gene discovery

期刊名称：EUPHYTICA （ 影响因子：2.185； 五年影响因子：2.387 ）

ISSN： 0014-2336

年卷期： 2022 年 218 卷 4 期

页码：

收录情况： SCI

摘要： Head splitting is a major physiological disease in cabbage. The most effective approach for controlling head splitting is to deploy genetic resistance by breeding cabbage cultivars with stable resistance. However, the genetic mechanisms regulating head-splitting resistance in cabbage remain largely unknown. Here, we employed the whole-genome resequencing-based approach (QTL-seq) to discover genomic regions, candidate genes, and diagnostic markers for head-splitting resistance in an F-2 segregation population derived from a cross between two inbred lines G274 (head-splitting susceptible) and G279 (head-splitting resistant). Single nucleotide polymorphism index (SNP-index) analysis indicated that one candidate genomic region spanning 5.6 Mb on chromosome 3 was responsible for head-splitting resistance. A candidate gene, Bol016058, was identified in this region, which is a homologue of proline-rich protein 4 (PRP4) and encodes a structural cell wall protein in Arabidopsis. This target gene was highly expressed in leaves, with four SNPs and one insertion-deletion (InDel) between G274 and G279. Moreover, a diagnostic InDel marker was successfully developed from this gene and validated on a set of breeding materials and could accelerate the selection of head-splitting resistant cabbage cultivars.

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