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A Set of Polymorphic Trinucleotide and Tetranucleotide Microsatellite Markers for Silver Crucian Carp (Carassius auratus gibelio) and Cross-Amplification in Crucian Carp

文献类型： 外文期刊

作者： Zheng, X. H. 1 ; Lu, C. Y. 1 ; Zhao, Y. Y. 1 ; Lee, C. 1 ; Cao, D. C. 1 ; Chang, Y. M. 1 ; Liang, L. Q. 1 ; Sun, X. W. 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Harbin 150070, Peoples R China

2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Carassius auratus gibelio;Microsatellites;Trinucleotides;Tetranucleotides

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期： 2010 年 48 卷 7-8 期

页码：

收录情况： SCI

摘要： Silver crucian carp (Carassius auratus gibelio Bloch), as a gynogenetic fish, is a promising model for the study of the evolutionary genetics of vertebrates. We have developed 59 polymorphic trinucleotide and tetranucleotide markers for the silver crucian carp through the biotin capture method and radioactive-labeling hybridization. The number of alleles ranged from 2 to 12 in the population, and the average proportion of heterozygotes (including tri- and diallelic) at polymorphic loci was 76.8%. In addition, these loci were successfully applied to a close relative, the crucian carp (Carassius auratus), by cross-amplification, as shown by the range of alleles (2-19), observed heterozygosity (0.1765-0.9706), expected heterozygosity (0.2392-0.9421), and polymorphism information content (0.2186-0.9236).

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