您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The Erk1/2-EGR1 signaling pathway is involved in lipopolysaccharide-induced transforming growth factor-beta 1 expression in mouse macrophages

文献类型： 外文期刊

作者： Zhao, Weimin 1 ; Wang, Hong 1 ; Dai, Chaohui 1 ; Li, Bixia 1 ; Fu, Yanfeng 1 ; Cheng, Jinhua 1 ; Li, Hui 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Jiangsu Prov Engn Res Ctr Precis Anim Breeding, Jiangsu Key Lab Food Qual & Safety,State Key Lab C, Sa, Nanjing 210014, Peoples R China

2.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China

关键词： LPS; TGF-beta 1; Erk1/2; EGR1; Macrophage

期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.5； 五年影响因子：3.6 ）

ISSN： 0882-4010

年卷期： 2025 年 203 卷

页码：

收录情况： SCI

摘要： Numerous studies have demonstrated that lipopolysaccharide (LPS) stimulates TGF-beta 1 expression. Although studies have implicated the NF-kappa B/METTL3/METTL14 transactivation/m6A-dependent and AMPK-dependent signaling pathways are engaged in this process in a variety of cell types, the underlying regulatory mechanism in murine macrophages is still not fully understood. To address this issue, in vitro studies were performed using the murine macrophage cell line, RAW264.7. The results showed that LPS challenge resulted in a significant increase in TGF-beta 1 expression at both mRNA and protein levels. Subsequent studies revealed that the MAPK (including p38, Erk1/2, and JNK) and NF-kappa B signaling pathways were activated in response to LPS stimulation, but only blocking the Erk1/2 singling pathway completely abolished LPS-induced TGF-beta 1 expression. Further studies revealed that the levels of a downstream regulator of the Erk1/2 pathway, EGR1, were significantly increased after LPS treatment, and its knockdown significantly reduced LPS-induced Tgf-131 expression levels. Finally, dual luciferase reporter and ChIP-PCR assays confirmed that EGR1 is a key transcription factor in the regulation of Tgf-131 expression by binding to its promoter region in response to LPS stimulation. In conclusion, we elucidated the molecular events by which LPS regulates TGF-beta 1 expression in murine macrophages through the Erk1/2-EGR1 signaling pathway. These findings provide a conceptually novel pathway for LPS-induced TGF beta 1 expression beyond the known NF-kappa B/METTL3/METTL14 transactivation/m6A-dependent and AMPKdependent signaling pathways.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台