Artificial gynogenesis in Cynoglossus semilaevis with homologous sperm and its verification using microsatellite markers
文献类型: 外文期刊
作者: Ji, Xiang-Shan 2 ; Tian, Yong-Sheng 1 ; Yang, Jing-Feng 1 ; Wu, Peng-Fei 1 ; Jiang, Yun-Liang 2 ; Chen, Song-Lin 1 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Shandong Agr Univ, Coll Anim Sci & Technol, Tai An, Shandong, Peoples R China
关键词: Cynoglossus semilaevis;gynogenesis;ploidy analyser;microsatellite
期刊名称:AQUACULTURE RESEARCH ( 影响因子:2.082; 五年影响因子:2.415 )
ISSN: 1355-557X
年卷期: 2010 年 41 卷 6 期
页码:
收录情况: SCI
摘要: Effective methods for induction of gynogenetic diploids in Cynoglossus semilaevis are needed to initiate monosex culture. An effective protocol to induce half-smooth tongue sole gynogenesis using homologous sperm was developed in this study. A UV dose of 50 mJ cm-2 was found to be the most effective for genetic inactivation of tongue sole sperm. Treatment optima for cold shocks were 5 degrees C for 20-23 min at 5 min after fertilization and the hatching rate of gynogenetic diploids was 10.0%. Microsatellite analysis at locus Csou 6 revealed that there was no genetic contribution from the paternal genome in 24 progenies of a meiotic gynogenetic family. Polymerase chain reaction demonstrated that only four individuals of 24 meiotic gynogenetic diploids produced the female-specific band of about 205 bp. The female/male ratio of gynogenetic diploids was significantly different from the theoretical ratio of 1:1. It is possible that there are some recessive lethal genes in W chromosome.
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