Development of Novel Wheat-Aegilops longissima 3S(1) Translocations Conferring Powdery Mildew Resistance and Specific Molecular Markers for Chromosome 3S(1)
文献类型: 外文期刊
作者: Li, Huanhuan 1 ; Tian, Xiubin 1 ; Pei, Shaolong 1 ; Men, Wenqiang 1 ; Ma, Chao 1 ; Sehgal, Sunish K. 2 ; Zhao, Yue 1 ; Chen, Qifan 1 ; Wang, Beilin 1 ; Dong, Zhenjie 1 ; Xiang, Zhiguo 3 ; Friebe, Bernd 4 ; Liu, Wenxuan 1 ;
作者机构: 1.Henan Agr Univ, Coll Life Sci, Zhengzhou 450002, Peoples R China
2.South Dakota State Univ, Dept Agron Hort & Plant Sci, Brookings, SD 57007 USA
3.Henan Acad Agr Sci, Zhengzhou 450002, Peoples R China
4.Kansas State Univ, Wheat Genet & Genom Resources Ctr, Throckmorton Plant Sci Ctr, Dept Plant Pathol, Manhattan, KS 66506 USA
关键词: Aegilops longissima; common wheat; molecular markers; powdery mildew resistance; 3S(1)
期刊名称:PLANT DISEASE ( 影响因子:4.614; 五年影响因子:5.33 )
ISSN: 0191-2917
年卷期: 2021 年 105 卷 10 期
页码:
收录情况: SCI
摘要: Y Powdery mildew of wheat, caused by Blumeria graminis f. sp. tritici, is a destructive disease of common wheat. Cultivation of resistant varieties is the most cost-effective disease management strategy. Previous studies reported that chromosome 3S(1)#2 present in Chinese Spring (CS)-Aegilops longissima 3Sl #2(3B) disomic substitution line TA3575 conferred resistance to powdery mildew. In this study, we further located the powdery mildew resistance gene(s) to the short arm of chromosome 3S(1)#2 (3S(1)#2S) by evaluating for B. graminis f. sp. tritici resistance of newly developed CS-Ae. longissima 3S(1)#2 translocation lines. Meanwhile, TA7545, a previously designated CS-Ae. longissima 3S(1)#3 disomic addition line, was reidentified as an isochromosome 3S(1)#3S addition line and evaluated to confer resistance to powdery mildew, thus locating the resistance gene(s) to the short arm of chromosome 3S(1)#3 (3S(1)#3S). Based on transcriptome sequences of TA3575, 10 novel chromosome 3S(1) S-specific markers were developed, of which 5 could be used to distinguish between 3S(1)#2S and 3S(1)#3S derived from Ae. longissima accessions TL20 and TA1910 (TAM4) and the remaining 5 could identify both 3S(1)#2S and 3S(1)#3S. Also, CL897, one of five markers specific to both 3S(1)#2S and 3S(1)#3S, could be used to detect Pm13 located at chromosome 3S(1)#1S from Ae. longissima accession TL01 in diverse wheat genetic backgrounds. The powdery mildew resistance genes on chromosomes 3S(1)#2S and 3S(1)#3S, the CS-Ae. longissima 3S(1)#2 translocation lines, and the 3S(1) S-specific markers developed in this study will facilitate the transfer of B. graminis f. sp. tritici resistance genes into common wheat and provide new germplasm resources for powdery mildew resistance breeding.
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