Identification and characterization of a fungal cutinase-like enzyme CpCut1 from Cladosporium sp. P7 for polyurethane degradation
文献类型： 外文期刊
作者： Liu, Jiawei 1 ; Xin, Kaiyuan 1 ; Zhang, Tianyang 1 ; Wen, Yuan 1 ; Li, Ding 2 ; Wei, Ren 3 ; Zhou, Jie 1 ; Cui, Zhongli 5 ; Dong, Weiliang 1 ; Jiang, Min 1 ;
作者机构： 1.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, Key Lab Waste Plast Biocatalyt Degradat & Recyclin, Nanjing, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing, Peoples R China
3.Univ Greifswald, Inst Biochem, Dept Biotechnol & Enzyme Catalysis, Res Grp Plast Biodegradat Jr, Greifswald, Germany
4.Nanjing Tech Univ, State Key Lab Mat Oriented Chem Engn, Nanjing, Peoples R China
5.Nanjing Agr Univ, Coll Life Sci, Key Lab Agr Environm Microbiol, Minist Agr, Nanjing, Peoples R China
关键词： polyurethane; fungal cutinase; biodegradation; Cladosporium sp.; polymer recycling
期刊名称：APPLIED AND ENVIRONMENTAL MICROBIOLOGY （ 影响因子：4.4； 五年影响因子：5.0 ）
ISSN： 0099-2240
年卷期： 2024 年 90 卷 4 期
页码：
收录情况： SCI
摘要： Plastic degradation by biological systems emerges as a prospective avenue for addressing the pressing global concern of plastic waste accumulation. The intricate chemical compositions and diverse structural facets inherent to polyurethanes (PU) substantially increase the complexity associated with PU waste management. Despite the extensive research endeavors spanning over decades, most known enzymes exhibit a propensity for hydrolyzing waterborne PU dispersion (i.e., the commercial Impranil DLN-SD), with only a limited capacity for the degradation of bulky PU materials. Here, we report a novel cutinase (CpCut1) derived from Cladosporium sp. P7, which demonstrates remarkable efficiency in the degrading of various polyester-PU materials. After 12-h incubation at 55(degrees)C, CpCut1 was capable of degrading 40.5% and 20.6% of thermoplastic PU film and post-consumer foam, respectively, while achieving complete depolymerization of Impranil DLN-SD. Further analysis of the degradation intermediates suggested that the activity of CpCut1 primarily targeted the ester bonds within the PU soft segments. The versatile performance of CpCut1 against a spectrum of polyester-PU materials positions it as a promising candidate for the bio-recycling of waste plastics.
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