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Identification of male sex-specific markers using genome re-sequencing in the Chinese longsnout catfish Leiocassis longirostris

文献类型： 外文期刊

作者： Luo, Hui 1 ; Li, Yu 1 ; Zheng, Shuqing 2 ; Zhou, Jian 4 ; Zou, Xinxi 1 ; Li, Chuangju 5 ; Ye, Huan 5 ; Li, Zhe 1 ; Zhou, Chaowei 1 ; Lv, Guangjun 1 ; Xiao, Shijun 6 ; Ye, Hua 1 ;

作者机构： 1.Southwest Univ, Coll Fisheries, Key Lab Freshwater Fish Reprod & Dev, Minist Educ, Chongqing 402460, Peoples R China

2.Key Lab Aquat Sci Chongqing, Chongqing 400175, Peoples R China

3.Aquat Sci & Technol Innovat Alliance Chongqing, Chongqing 402460, Peoples R China

4.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Peoples R China

5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China

6.Jiaxing Key Lab New Germplasm Breeding Econ Mycol, Jiaxing 314000, Zhejiang, Peoples R China

关键词： Leiocassis longirostris; Sex-specific marker; Sex chromosome; Sex determining region

期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）

ISSN： 0044-8486

年卷期： 2022 年 558 卷

页码：

收录情况： SCI

摘要： The Chinese longsnout catfish (Leiocassis longirostris Gunther), an indigenous commercially important fish species in China, exhibits markedly sex dimorphism in growth, which the males grow much faster than females. So, we aimed to develop sex-specific DNA markers to accelerate the development of sex control techniques for L. longirostris. In this study, 15 individuals of each sex were sequenced using whole-genome re-sequencing technology. A total of 238.51 Gb clean data was used to detect the sex associated markers and we found that 2472 loci were enriched in the first 18 Mb of Chr 7, the putative sex-determination region for L. longirostris. All candidate markers were male heterozygous, implying that the sex-determination system of the L. longirostris is the XX/XY type. Using the sequence polymorphism of male and female individuals in this region, we designed six pairs of primers to develop sex-specific markers, of which three markers exclusively exhibited male-specific bands. Further validation showed that the accuracy of sex prediction was up to 100% for individuals in the Meishan, Chengdu, Yibin and Shishou regions, suggesting wide applicability among local populations. Importantly, marker 3 was located in the ACAP1 gene, which may be involved in sex determination or differentiation of L. longirostris. This method for developing sex-specific markers is efficient, simple, and provides a useful reference that can be applied to other fish species. The markers developed in this work could be instrumental for sex control breeding to increase the yield in the L. longirostris aquaculture industry.

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