Isolation and characterization of sex-linked SNP markers from transcriptomic sequences of the Chinese soft-shelled turtle (Pelodiscus sinensis)
文献类型： 外文期刊
作者： Li, Wei 1 ; Zhu, Junxian 1 ; Chen, Chen 1 ; Wang, Yakun 1 ; Lei, Luo 1 ; Geng, Lulu 1 ; Su, Junyu 1 ; Zhu, Xinping 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Trop & Subtrop Fishery Resource Applicat, Minist Agr & Rural Affairs, Guangzhou 510380, Guangdong, Peoples R China
2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 21408, Jiangsu, Peoples R China
3.Foshan Univ, Coll Life Sci & Engn, Foshan 528011, Guangdong, Peoples R China
关键词： Chinese soft-shelled turtle (Pelodiscus sinensis); Sex-linked marker; Single nucleotide polymorphism (SNP); Transcriptome
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.991； 五年影响因子：0.897 ）
ISSN： 1877-7252
年卷期： 2022 年 14 卷 2 期
页码：
收录情况： SCI
摘要： The Chinese soft-shelled turtle (Pelodiscus sinensis) exhibits sexual dimorphism in adults and the absence of sexually dimorphic morphological characteristics in the long-lived juvenile stage. The development of sex-linked SNP markers is of great significance for the improvement and replenishment of genetic resources. Here, we developed and characterized 35 novel polymorphic SNP markers based on the gonadal transcriptome of P. sinensis. The polymorphism information content, observed heterozygosity and expected heterozygosity of the 35 SNP markers ranged from 0.023 to 0.473, 0.012 to 0.547 and 0.023 to 0.503, respectively, and 10 of the SNPs significantly deviated from Hardy-Weinberg equilibrium (PHWE < 0.01). Two sex-linked SNPs were found to be significantly associated with sex phenotypes and showed 100% concordance across all tested individuals. These novel polymorphic SNP markers will be useful for future genetic studies of P. sinensis.
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