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A polypeptide from Chlamys farreri inhibits UVB-induced HaCaT cells apoptosis via the Apaf-1/caspase-9 and Smac/XIAP signaling pathway

文献类型: 外文期刊

作者: Liu Xiaojin 1 ; Wang Wencheng 2 ; Wang Hongjiang 1 ; Zhang Lanlan 1 ; Liu Leqian 3 ; Wang Yuejun 4 ; Wang Chunbo 1 ;

作者机构: 1.Qingdao Univ, Coll Med, Dept Pharmacol, Qingdao 266071, Peoples R China

2.Taishan Univ, Coll Med, Tai An 271000, Shandong, Peoples R China

3.E Tennessee State Univ, Dept Biol Sci, Johnson City, TN 37614 USA

4.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词: polypeptide from Chlamys farreri (PCF);UVB;apoptosis;Smac/XIAP;Apaf-1/caspase-9

期刊名称:CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY ( 影响因子:1.068; 五年影响因子:0.983 )

ISSN: 0254-4059

年卷期: 2009 年 27 卷 3 期

页码:

收录情况: SCI

摘要: A novel marine active polypeptide (PCF), isolated from the gonochoric Chinese scallop, Chlamys farreri, has potential antioxidant and anti-apoptotic activity against ultraviolet irradiation. We investigated whether UVB-induced HaCaT cell apoptosis occurs via the mitochondrial pathways Apaf-1/caspase-9 and Smac/XIAP/caspase-3. We then investigated the molecular mechanisms controlling the anti-apoptotic effect of PCF Pre-treatment with PCF and caspase-9 inhibitor significantly inhibited UVB-induced apoptosis in HaCaT cells based on a DNA fragmentation assay and Hoechst 33258 staining. The expression of Apaf-1 and the cleavage of procaspase-9 were dose-dependently reduced by 1.42-5.96 mmol/L PCF pretreatment in UVB-irradiated HaCaT cells. This was followed by inhibition of cleavage of procaspase-3, whose activation induced cell apoptosis. Meanwhile, PCF significantly and dose-dependently enhanced the activation of ATPase. Furthermore, we demonstrated that PCF strongly inhibited the release of Smac from the mitochondria to cytosol by reducing the degradation of XIAP dose-dependently. We conclude that the protective effect of PCF against UVB irradiation in HaCaT cells may be attributed to the inhibition of the Apaf-1/caspase-9 and Smac/XIAP/caspase-3 apoptotic signaling pathways.

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