Effects of cryoprotectants and salinity on viability and fatty acid profile of two haptophytes during preservation at low temperature (-22 +/- 1 degrees C)
文献类型: 外文期刊
作者: Zhu, Bao H. 1 ; Ye, Nai H. 3 ; Pan, Ke H. 1 ; Lin, Li M. 2 ;
作者机构: 1.Ocean Univ China, Coll Fisheries, Key Lab Mariculture, Minist Educ, Qingdao 266003, Peoples R China
2.Shandong Entry Exit Inspect & Quarantine, Qingdao 266002, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词: Pavlova viridis;Isochrysis galbana Parke;Cryoprotectants;Salinity;Viability;Fatty acids
期刊名称:CAHIERS DE BIOLOGIE MARINE ( 影响因子:0.527; 五年影响因子:0.67 )
ISSN: 0007-9723
年卷期: 2009 年 50 卷 1 期
页码:
收录情况: SCI
摘要: Aquaculture production could be improved by using preserved microalgal biomass as feedstuff for marine animal larvae and juveniles. The present paper reports the effects of different kinds of cryoprotectants and salinities on the viability and fatty acid profile of the two haptophytes Pavlova viridis and Isochrysis galbana Parke during preservation at -22 +/- 1 degrees C. The best protectants of the two haptophytes were selected by evaluating their post-thaw viability. Results indicate that 10% DMSO (dimethyl-sulfoxide) + 5% Glycerol and 10% DMSO + 5% Proline are the best protectants for Pavlova viridis and Isochrysis galbana Parke, respectively. These two haptophytes displayed a better viability at the higher salinities of 21.3 and 32, respectively. Furthermore, the fatty acid profile on a total fatty acid basis remained stable over storage time, irrespective of viability. This is an important fact to bear in mind, as fatty acids especially for PUFAs play an important role in the quality of the cell as food in aquaculture.
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