Molecular characterization and functional analysis of DMRT11E in black tiger shrimp (Penaeus monodon)
文献类型： 外文期刊
作者： Wei, Wen-Ya 1 ; Huang, Jian-Hua 1 ; Yang, Qi-Bin 4 ; Zhou, Fa-Lin 1 ; Jiang, Song 1 ; Li, Yun-Dong 1 ; Jiang, Shi-Gui 1 ; Yang, Li-Shi 1 ;
作者机构： 1.Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Peoples R China
2.Chinese Acad Fishery Sci, Shenzhen Base South China Sea Fisheries Res Inst, Shenzhen 518108, Peoples R China
3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China
4.Chinese Acad Fishery Sci, Trop Aquaculture Res & Dev Ctr, South China Sea Fisheries Res Inst, Sanya 572018, Peoples R China
5.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, 231 Xingang West Rd, Guangzhou 510300, Peoples R China
关键词： Penaeus monodon; DMRT11E; DM domain; Sexual differentiation; RNA interference
期刊名称：AQUACULTURE REPORTS （ 影响因子：3.385； 五年影响因子：3.645 ）
ISSN： 2352-5134
年卷期： 2022 年 22 卷
页码：
收录情况： SCI
摘要： As a highly conserved transcription factor family, DMRTs (Doublesex and Mab-3-related transcription factors) are involved in sex determination and sexual differentiation in numerous animals. In the present study, we identified a DM (Doublesex/Mab-3)-domain gene in Penaeus monodon, which we named PmDMRT11E because it shared many similarities with DMRT11E homologs and has a close relationship in the phylogeny of different species. The three-dimensional structure and amino acid alignments indicated the stability and conservation of the DM domain. Real-time PCR analysis revealed that PmDMRT11E was highly and preferentially expressed in the testis of sub-adult shrimp. During embryogenesis, the expression level of PmDMRT11E was higher at the nauplius than at other periods. PmDMRT11E dsRNA injection significantly decreased the transcription factor Sox9 and increased ovarian marker Foxl2 expression, implying its important regulatory role in the mechanism of male determination. Moreover, dsDMRT11E caused abnormal sperm development, indicating that PmDMRT11E may be closely related to gonadal development and spermatogenesis.
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