Purification, characterization and anti-hyperuricemic mechanism of novel xanthine oxidase inhibitory peptides from tea (Camellia sinensis L.) protein
文献类型： 外文期刊
作者： Ma, Feng 1 ; Sun, Shili 2 ; Ye, Haoduo 1 ; Zhang, Zhenyu 1 ; Chen, Qimiao 1 ; Yin, Shouwei 3 ; Cao, Yong 1 ; Miao, Jianyin 1 ;
作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Peoples R China
2.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Prov Key Lab Tea Plant Resources Innova, Guangzhou 510640, Peoples R China
3.South China Univ Technol, Res & Dev Ctr Food Prot, Dept Food Sci & Technol, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Guangzhou 51640, Peoples R China
关键词： Tea ( Camellia sinensis L.) protein; Xanthine oxidase inhibitory peptides; Anti-hyperuricemic activity; Molecular docking; Hyperuricemia cell model; Activity mechanism
期刊名称：FOOD BIOSCIENCE （ 影响因子：4.8； 五年影响因子：5.1 ）
ISSN： 2212-4292
年卷期： 2024 年 61 卷
页码：
收录情况： SCI
摘要： In this study, two novel xanthine oxidase inhibitory peptides, PDEAVAYG (820.3602 Da) and IAAGLQNTG (843.4450 Da), were purified and identified from tea protein hydrolysate, and their IC50 values were 0.09 mg/mL (109.71 mu M) and 0.24 mg/mL (284.55 mu M), respectively. During the gastrointestinal simulation digestion, PDEAVAYG was broken down into new peptides, while IAAGLQNTG exhibited some stability. Molecular docking results showed that hydrogen bonding, Ic-Ic stacking, and hydrophobic interactions exerted crucial effects on the interaction between peptides and xanthine oxidase. In the hyperuricemia cell model, compared to the model group, 1.0 mg/mL of PDEAVAYG and IAAGLQNTG decreased cellular uric acid levels by 40.80% and 33.33%, respectively. The RNA-seq experiments revealed that PDEAVAYG could alleviate hyperuricemia by regulating mRNA expression for pro-inflammatory factors, growth factors associated with cardiovascular disease, and uric acid efflux transporter proteins in cells. This study provides a new theoretical reference for the development of functional foods or nutritional supplements using peptides with anti-hyperuricemic activity.
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