文献类型： 外文期刊

作者： Zhu, Shuangju ¹ ; Tang, Yue ¹ ; Shi, Bin ² ; Zou, Wenying ¹ ; Wang, Xueli ¹ ; Wang, Chunxiao ¹ ; Wu, Yuangen ¹ ;

作者机构： 1.Guizhou Univ, Sch Liquor & Food Engn, Guizhou Prov Key Lab Fermentat Engn & Biopharm, Guiyang 550025, Peoples R China

2.Guizhou Acad Agr Sci, Guizhou Res Inst Modern Agr Dev, Guiyang 550006, Peoples R China

3.China Natl Light Ind, Key Lab Wuliangye Flavor Liquor Solid State Ferme, Yibin 644000, Peoples R China

关键词： Food-borne pathogens; Metal oxides; Oxidase-liking activity; Aptamer; Nanozyme

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：9.221； 五年影响因子：7.676 ）

ISSN：

年卷期： 2021 年 349 卷

页码：

收录情况： SCI

摘要： Colorimetric detection of pathogenic bacteria in food still faces many challenges. Herein, a novel label-free and ultrasensitive colorimetric aptasensor was firstly established for the detection of food-borne pathogenic like Staphylococcus aureus (S. aureus) based on oligonucleotide-mediated the oxidase-mimicking activity of octahedral Mn3O4 nanoparticles (NPs). The study confirmed that the common oligonucleotide such as SA31 aptamer can adsorb on the surface of octahedral Mn3O4. NPs, and significantly inhibit the oxidase-mimicking activity by blocking their electron transfer to 3,3',5,5'-tetramethylbenzidine. In the presence of S. aureus, aptamers will preferentially bind to bacterial cell, resulting in the recovery of oxidase-mimicking activity. The naked eye can easily recognize the color change from green to yellow, with the increase concentration of S. aureus from 10 CFU.mL(-1) to 2 x 10(5) CFU.mL(-1). The colorimetric aptasensor shows a low detection limit of 3 CFU.mL(-1) (S/N = 3). This strategy has excellent selectivity whether the presence of other competitive bacteria, and has been successfully applied to the non-enrichment detection of S. aureus in milk and pork samples.