Proline-Nitrogen Metabolic Coordination Mediates Cold Priming-Induced Freezing Tolerance in Maize
文献类型： 外文期刊
作者： Gai, Zhijia 1 ; Liu, Lei 2 ; Zhang, Na 1 ; Liu, Jingqi 1 ; Cai, Lijun 1 ; Yang, Xu 1 ; Zhang, Ao 3 ; Zhang, Pengfei 4 ; Ding, Junjie 1 ; Zhang, Yifei 3 ;
作者机构： 1.Heilongjiang Acad Agr Sci, Jiamusi Branch, Jiamusi 154007, Peoples R China
2.Jilin Agr Univ, Coll Resources & Environm, Changchun 130102, Peoples R China
3.Heilongjiang Bayi Agr Univ, Coll Agron, Daqing 163319, Peoples R China
4.Northeast Agr Univ, Coll Agron, Harbin 150030, Peoples R China
关键词： photosynthesis; proline metabolism; antioxidant enzyme; GDH/ICDH; osmoregulation; ROS
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Cold stress critically restricts maize seedling growth in Northeast China, yet the mechanism by which cold priming (CP) enhances cold tolerance through proline-nitrogen metabolic networks remains unclear. This study systematically investigated CP's synergistic regulation in cold-tolerant (Heyu27) and cold-sensitive (Dunyu213) maize using a two-phase temperature regime (priming induction/stress response) with physiological and multivariate analyses. CP alleviated cold-induced photosynthetic inhibition while maintaining a higher chlorophyll and photosynthetic rate, though biomass responses showed varietal specificity, with Heyu27 minimizing growth loss through optimized carbon-nitrogen allocation. Antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were pre-activated during early stress, effectively scavenging reactive oxygen species (ROS) and reducing malondialdehyde (MDA) accumulation, with Heyu27 showing superior redox homeostasis. CP enhanced proline accumulation via bidirectional enzyme regulation (upregulating triangle 1-pyrroline-5-carboxylate synthase/reductase [P5CS/P5CR], inhibiting proline dehydrogenase [ProDH]) and reprogrammed nitrogen metabolism through glutamate dehydrogenase/isocitrate dehydrogenase (GDH/ICDH)-mediated ammonium conversion to glutamate, alleviating nitrogen dysregulation while supplying proline precursors. Principal component analysis revealed divergent strategies: Heyu27 prioritized proline-antioxidant synergy, whereas Dunyu213 emphasized photosynthetic adjustments. These findings demonstrate that CP establishes "metabolic memory" through optimized proline-nitrogen coordination, synergistically enhancing osmoregulation, reactive oxygen species (ROS) scavenging, and nitrogen utilization. This study elucidates C4-specific cold adaptation mechanisms, advancing cold-resistant breeding and stress-resilient agronomy.
- 相关文献
作者其他论文 更多>>
-
Identification, Characterization, and Chemical Management of Fusarium asiaticum Causing Soybean Root Rot in Northeast China
作者：Liu, Jinxin;Cui, Wanqiu;Zhao, Qingyi;Li, Yonggang;Ren, Zhipeng;Li, Lin;Sun, Lei;Ding, Junjie
关键词：soybean; root rot;
Fusarium asiaticum ; host range; identification; fungicide efficacy -
Cadmium contamination impairs alkaline tolerance in sugar beet by inhibiting carbon fixation and tryptophan metabolism
作者：Ye, Shuo;Zhou, Xinrui;Tang, Fawei;Qu, Ruixin;Wang, Zhiwei;Hu, Xu;Liu, Yang;Li, Donglin;Liu, Lei;Gai, Zhijia;Li, Xiangnan;Liu, Lei;Zhang, Pengfei;Yang, Kepan;Yang, Kepan
关键词：Alkaline; Cadmium; Carbohydrate metabolism; Combined stresses; Melatonin
-
Optimized design and experimental analysis of the vertical mixing plow for enhancing planosol improvement machine
作者：Du, Jiaxing;Hou, Jiyao;Dun, Yuanyuan;Yang, Chuanhua;Li, Xiaohai;Zhu, Baoguo;Cai, Lijun;Dun, Guoqiang
关键词：Subsoil compaction; Low nutrient; Root penetration; Tillage depth; Discrete element simulation; Mixing rate
-
Comprehensive evaluation of rice quality in response to nitrogen application
作者：Su, Xinying;Xu, Shuang;Xu, Zhong;Zhang, Na;Cao, Liangzi;Cao, Liangzi;Wang, Li
关键词：
-
Identification and function analysis of drought-responsive miRNAs in sorghum (Sorghum bicolor)
作者：Ma, Junming;Liu, Zhuojun;Guo, Zihao;Wang, Xinyu;Zou, Chunlei;Zhang, Chunlai;Gai, Zhijia
关键词：GO term; Non-coding RNA; Stress tolerance; Target gene; Water-deficiency
-
Fine Mapping of QTLs/QTNs and Mining of Genes Associated with Race 7 of the Soybean Cercospora sojina by Combining Linkages and GWAS
作者：Liu, Yanzuo;Hu, Bo;Yu, Aitong;Liu, Yuxi;Xu, Pengfei;Zhang, Shuzhen;Li, Wen-Xia;Ning, Hailong;Wang, Yang;Ding, Junjie
关键词：soybean; cultivar leaf spot; QTL; QTN; resistance gene
-
Alkaline stress suppresses soybean waterlogging tolerance by exacerbating energy expenditure and ROS accumulation
作者：Liu, Tianhao;Zhang, Peng;Li, Shuxin;Liu, Lei;Li, Zhilong;Wang, Zhiwei;Ye, Shuo;Liu, Lei;Gai, Zhijia;Wang, Ziyang;Yang, Kepan;Yang, Kepan
关键词：Waterlogging; Saline-alkali; Combined stress; Reactive oxygen species; Anaerobic respiration
