您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Development of a rapid and sensitive reverse transcription real-time quantitative PCR assay for detection and quantification of grass carp reovirus II

文献类型： 外文期刊

作者： Li, Jiahao 1 ; Wu, Huiliang 2 ; Xu, Wei 3 ; Wang, Yajun 1 ; Wang, Hao 3 ; Wang, Yingying 1 ; Li, Yingying 1 ; Shi, Cunbin 1 ; Bergmann, Sven M. 4 ; Mo, Xubing 1 ; Wang, Qing 1 ; Yin, Jiyuan 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev ,Key Lab Aquat Anim Immun, Guangzhou, Guangdong, Peoples R China

2.South China Agr Univ, Coll Vet Med, Guangzhou, Peoples R China

3.Shanghai Ocean Univ, Natl Pathogen Collect Ctr Aquat Anim, Shanghai 201306, Peoples R China

4.Fed Res Inst Anim Hlth, Friedrich Loeffler Inst FLI, Inst Infectol, Greifswald, Germany

5.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China

关键词： GCRV-II; Quantitative analysis; RT-qPCR; Detection

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：3.1； 五年影响因子：2.4 ）

ISSN： 0166-0934

年卷期： 2023 年 312 卷

页码：

收录情况： SCI

摘要： Hemorrhagic disease of grass carp, which is induced by grass carp reovirus II (GCRV-II), leads to mass mortality in grass carp culture and causes enormous economic loss. However, there is currently no quantitative analysis method for the detection of GCRV-II, which is greatly restricted the etiological and epidemiological study of the disease. In this study a reverse transcription TaqMan PCR (RT-qPCR) assay was developed for the quantitative detection of GCRV-II. The probe and primers targeted location is the segment 6 (S6) region of the GCRV-II genome which is highly conserved. Standard curves were drawn and criteria were confirmed after the deter-mination of the optimum reaction conditions. The species-specific assay showed that the method is highly specific and has no cross reactions with other pathogens. The assay was sufficiently sensitive to detect as low as 10 copies of virus RNA. Moreover, the method has a very good repeatability for batches and inter-batches sample detection. Then the method was applied to detect the virus in tissue samples from clinically infected grass carp, compared with conventional RT-seminested PCR, the RT-qPCR represents a specific value for detection rate of positive samples. In summary, the RT-qPCR was applied and achieved high sensitivity and specificity for GCRV-II detection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台