文献类型: 外文期刊
作者: Wang, Wei 1 ; Sun, Mi 2 ; Liu, Wan-Shun 1 ;
作者机构: 1.Ocean Univ China, Coll Marine Life Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
关键词: Antarctic surface seawater;psychrophilic catalase;Bacillus;isolation;identification
期刊名称:ANNALS OF MICROBIOLOGY ( 影响因子:2.112; 五年影响因子:2.151 )
ISSN: 1590-4261
年卷期: 2008 年 58 卷 1 期
页码:
收录情况: SCI
摘要: Forty-nine colonies were isolated from Antarctic surface seawater sample inoculated on Marine agar plates containing 1.45 mu M H2O2. These bacteria were all endospore-forming, strictly aerobic and motile rods. Their cell extracts showed high catalase activity of 1200 295 U/mg. High activity of the catalase at low temperatures was in accordance with the environmental conditions under which the microorganisms lived. Identification of the Antarctic seawater isolates by several morphological, biochemical and cultural methods showed that they belonged to the same species, among which the strain N2a was chosen as representative. This strain was determined to be a member of Bacillus according to its 16S rDNA and fatty acids profile. However, culturing properties (growth at 4 degrees C and in 6% NaCl) and relatively high unsaturated fatty acids (> 40%) of the strain N2a were different from those of the 16S rDNA phylogenetic relatives, exhibiting the characteristics of psychrotolerant marine bacteria. Thus these results supported the hypothesis that the Antarctic isolates were novel species of the genus Bacillus.
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