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Purification and properties of lysozyme from a marine strain

文献类型： 外文期刊

作者： Zhang, Xiu 1 ; Wang, Yue-Jun 2 ; Sun, Mi 2 ; Ren, Xian 1 ; Zou, Yan-Li 2 ; Wang, Qing-Yin 2 ; Wang, Wei 2 ;

作者机构： 1.N Univ Ethn, Coll Biol Sci & Engn, Yinchuan 750021, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： marine strain;purification;lysozyme

期刊名称：ANNALS OF MICROBIOLOGY （ 影响因子：2.112； 五年影响因子：2.151 ）

ISSN： 1590-4261

年卷期： 2008 年 58 卷 1 期

页码：

收录情况： SCI

摘要： An extracellular lysozyme from a new strain which was screened from a sea mud sample was purified to homogeneity by ultrafiltration, followed by ammonium sulphate precipitation, dialysis, Sephadex G-100 chromatography and CM-Sephadex-50 ion-exchange chromatography. This purification protocol resulted in a 65.28 fold purification of lysozyme with 17.68% final yield and the relative molecular weight of the enzyme was determined to be 16.0 kDa by SDS-PAGE. The kinetic characterisation of the purified enzyme exhibited maximum activity at 35 degrees C and pH 8.0. The enzyme activity was promoted in the presence of Zn2+ and Cu2+, and inhibited by Mn2+. EDTA did not affect the enzyme activity. The enzyme was highly stable in the presence of several thickening agents. Some surfactants increased its activity. It had a broad antimicrobial spectrum against several Gram positive bacteria, Gram negative bacteria and fungi. The enzyme has broad potential application.

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