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Revealing the contribution of GbPR10.5D1 to resistance against Verticillium dahliae and its regulation for structural defense and immune signaling

文献类型： 外文期刊

作者： Guo, Jin 1 ; Cao, Peihua 1 ; Yuan, Leitian 1 ; Xia, Guixian 3 ; Zhang, Huanyang 4 ; Li, Jing 4 ; Wang, Fuxin 1 ;

作者机构： 1.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China

2.Key Lab Microbial Divers Res & Applicat Hebei Pro, Baoding 071002, Peoples R China

3.Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

4.Shanxi Acad Agr Sci, Inst Cotton Res, Yuncheng 044000, Shanxi, Peoples R China

期刊名称：PLANT GENOME （ 影响因子：4.219； 五年影响因子：5.826 ）

ISSN：

年卷期： 2022 年 15 卷 4 期

页码：

收录情况： SCI

摘要： As an important family of pathogenesis-related (PR) proteins, the functional diversification and roles of PR10s in biotic stress have been well documented. However, the molecular basis of PR10s in plant defense responses against pathogens remains to be further understood. In the present study, we analyzed the phylogenetic relationship and function of a novel PR10 named GbPR10.5D1 in Sea-Island (or Pima or Egyptian) cotton (Gossypium barbadense L.), which has been identified as a Verticillium dahliae Kleb.-induced protein in a previous proteomics study. Phylogenetic analysis revealed that GbPR10.5D1, located on chromosome 2, is a unique member of GbPR10. The expression of GbPR10.5D1 was preferably in the root and induced upon V. dahliae infection. GbPR10.5D1 proteins were distributed in both nucleus and cytoplasm. GbPR10.5D1-virus-induced gene-silencing (VIGS) cotton plants were more susceptible to infection by V. dahliae, whereas overexpression (OE) of GbPR10.5D1 in cotton enhanced the resistance. By comparative transcriptome analysis between GbPR10.5D1-OE and wild-type (WT) plants and quantitative real-time polymerase chain reaction (qRT-PCR) verification, we found transcriptional activation of genes involved in cutin, suberine, and wax biosynthesis and mitogen-activated protein kinase (MAPK) signaling under normal conditions. Upon pathogen infection, defense signaling, fatty acid degradation, and glycerolipid metabolism were specifically activated in GbPR10.5D1-OE plants; biological processes (BPs), including glycolysis and gluconeogenesis, DNA replication, and cell wall organization, were specifically repressed in WT plants. Collectively, we proposed that GbPR10.5D1 possibly mediated lipid metabolism pathway to strengthen structural defense and activate defense signaling, which largely released the repression of cell growth caused by V. dahliae infection.

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