Induction of Triploid Grass Carp (Ctenopharyngodon idella) and Changes in Embryonic Transcriptome
文献类型： 外文期刊
作者： Zixuan, E. 1 ; Wen, Han 1 ; Tang, Yingshi 1 ; Zhang, Mingqing 1 ; Wang, Yaorong 1 ; Liao, Shujia 1 ; Chen, Kejun 1 ; Lu, Danqi 1 ; Lin, Haoran 1 ; Huang, Wen 3 ; Chen, Xiaoying 3 ; Zhang, Yong 1 ; Li, Shuisheng 1 ;
作者机构： 1.Sun Yat Sen Univ, State Key Lab Biocontrol, Guangzhou 510275, Peoples R China
2.Sun Yat Sen Univ, Guangdong Prov Engn Technol Res Ctr Hlth Breeding, Southern Marine Sci & Engn Guangdong Lab Zhuhai, Inst Aquat Econ Anim,Sch Life Sci,Guangdong Lab Zh, Guangzhou 510275, Peoples R China
3.Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed Sci South China, Guangdong Prov Key Lab Anim Breeding & Nutr, Inst Anim Sci,Minist Agr & Rural Affairs, Guangzhou 510640, Peoples R China
4.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China
关键词： grass carp; triploidy; cold shock; embryonic transcriptome
期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）
ISSN： 2076-2615
年卷期： 2025 年 15 卷 15 期
页码：
收录情况： SCI
摘要： Grass carp is an economically important cultured species in China. Triploid embryo production is widely applied in aquaculture to achieve reproductive sterility, improve somatic growth, and reduce ecological risks associated with uncontrolled breeding. In this study, a simple cold shock method for inducing triploid grass carp was developed. The triploid induction rate of 71.73 +/- 5.00% was achieved by applying a cold treatment at 4 degrees C for 12 min, starting 2 min after artificial fertilization. Flow cytometry and karyotype analysis revealed that triploid individuals exhibited a 1.5-fold increase in DNA content compared to diploid counterparts, with a chromosomal composition of 3n = 72 (33m + 36sm + 3st). Additionally, embryonic transcriptome analysis demonstrated that, in the cold shock-induced embryos, genes associated with abnormal mesoderm and dorsal-ventral axis formation, zygotic genome activation (ZGA), and anti-apoptosis were downregulated, whereas pro-apoptotic genes were upregulated, which may contribute to the higher abnormal mortality observed during embryonic development. Overall, this study demonstrates optimized conditions for inducing triploidy in grass carp via cold shock and provides insights into the transcriptomic changes that take place in cold shock-induced embryos, which could inform future grass carp genetic breeding programs.
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