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Transcriptome Analysis of Metapenaeus affinis Reveals Genes Involved in Gonadal Development

文献类型： 外文期刊

作者： Zhang, Yan 1 ; Li, Yun-Dong 2 ; Yang, Qi-Bin 2 ; Jiang, Song 2 ; Jiang, Shi-Gui 2 ; Zhou, Fa-Lin 2 ;

作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Minist Agr & Rural Affairs, Key Lab South China Sea Fishery Resources Exploit, 231 Xingang West Rd, Guangzhou 510300, Peoples R China

关键词： DEGs; Metapenaeus affinis; Molecular mechanism; Ovary; RNA-Seq

期刊名称：ISRAELI JOURNAL OF AQUACULTURE-BAMIDGEH （ 影响因子：0.6； 五年影响因子：0.6 ）

ISSN： 0792-156X

年卷期： 2022 年 74 卷

页码：

收录情况： SCI

摘要： Metapenaeus affinis is a crustacean with important commercial value in the fishery of the South China Sea. Overfishing has resulted in the decline of the wild population and germplasm degradation. However, there is little background knowledge about its gonadal development, and there is a lack of research on the development of this species. To better understand the molecular regulatory mechanisms during gonadal development, here, we performed RNA-Seq on immature and mature ovaries and compared their transcriptomic signatures. 126,930,488 and 122,677,356 clean sequencing reads were obtained from the Illumina sequencing platform, respectively. 394 differentially expressed genes (DEGs) were identified, of which 136 were up-regulated, and 258 were downregulated. Further analysis revealed rich transcriptional sequences, which have homology with genes related to reproduction and development. Expression patterns of COX, GPX, E3s, PCNA, STPK, and other genes were changed during ovarian development. Validation by qRT-PCR demonstrated the reliability of RNA-Seq. This study has made a significant contribution to the currently available sequence data of M. affinis and provided reference data for the development of genetic and breeding work.

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