Rapid detection of the phytopathogenic oomycete Phytopythium helicoides with a visualized loop-mediated isothermal amplification assay
文献类型： 外文期刊
作者： Ren, Le-Yao 1 ; Wen, Ke 1 ; Cheng, Bao-Ping 2 ; Jin, Jing-Hao 1 ; Srivastava, Vaibhav 3 ; Chen, Xiao-Ren 1 ;
作者机构： 1.Yangzhou Univ, Coll Plant Protect, 48 Eastern Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China
2.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Guangdong, Peoples R China
3.AlbaNova Univ Ctr, KTH Royal Inst Technol, Sch Engn Sci Chem, Dept Chem,Div Glycosci, Stockholm, Sweden
关键词：
期刊名称：BRAZILIAN JOURNAL OF MICROBIOLOGY （ 影响因子：1.9； 五年影响因子：2.4 ）
ISSN： 1517-8382
年卷期： 2025 年 56 卷 1 期
页码：
收录情况： SCI
摘要： The phytopathogenic oomycete Phytopythium helicoides, previously known as Pythium helicoides, has emerged as a new threat to the Shatangju citrus cultivar (Citrus reticulata cv. Shatangju; '(sic)(sic)(sic)' in Chinese) in southern China. To enable rapid diagnosis and control of the leaf blight and stem rot caused by P. helicoides, we developed a visualized loop-mediated isothermal amplification (LAMP) assay targeting the beta-tubulin gene of the pathogen. Using a carefully selected set of primers and optimized reaction conditions at 60 degrees C for 60 min, the LAMP assay can specifically detect the pathogen among various citrus-associated phytopathogens. The assay demonstrated high sensitivity, detecting as little as 30 pg of the pathogen DNA, comparable to the sensitivity of quantitative polymerase chain reaction (qPCR). We validated the practical application of this LAMP assay for diagnosing infections in citrus leaves and strawberry crowns. This newly developed LAMP assay offers a specific, sensitive, and rapid detection tool for P. helicoides, aiding in the control of Shatangju citrus leaf blight and stem rot, as well as other related crop diseases like strawberry crown rot.
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