您好，欢迎访问黑龙江省农业科学院 机构知识库！
黑龙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

I3-sitosterol alleviates high fatty acid-induced lipid accumulation in calf hepatocytes by regulating cholesterol metabolism

文献类型： 外文期刊

作者： Yang, Wei 1 ; Tian, Yan 1 ; Yang, Mingmao 2 ; Mauck, John 3 ; Loor, Juan J. 3 ; Jia, Bin 1 ; Wang, Shuang 1 ; Fan, Wenwen 1 ; Li, Zhendong 1 ; Zhang, Bingbing 5 ; Xu, Chuang 1 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163319, Peoples R China

2.Northwest A&F Univ, Coll Vet Med, Key Lab Anim Biotechnol, Minist Agr, Xianyang 712100, Peoples R China

3.Univ Illinois, Dept Anim Sci, Div Nutr Sci, Mammalian Nutri Physio Genom, Urbana, IL USA

4.Heilongjiang Acad Agr Sci, Branch Anim Husb & Vet, Qiqihar 163005, Peoples R China

5.Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China

6.China Agr Univ, Coll Vet Med, Beijing 100193, Peoples R China

关键词： I3-sitosterol; Primary calf hepatocytes; Cholesterol metabolism; Oxidative stress; Fatty liver

期刊名称：JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：2.7； 五年影响因子：3.6 ）

ISSN： 0960-0760

年卷期： 2024 年 243 卷

页码：

收录情况： SCI

摘要： A significant reduction in plasma concentration of cholesterol during early lactation is a common occurrence in high-yielding dairy cows. An insufficient synthesis of cholesterol in the liver has been linked to lipid accumulation caused by high concentrations of fatty acids during negative energy balance (NEB). As ruminant diets do not provide quantitative amounts of cholesterol for absorption, phytosterols such as I3 -sitosterol may serve to mitigate the shortfall in cholesterol within the liver during NEB. To gain mechanistic insights, primary hepatocytes were isolated from healthy female 1-day old calves for in vitro studies with or without 1.2 mM fatty acids (FA) to induce metabolic stress. Furthermore, hepatocytes were treated with 50 mu M I3 -sitosterol with or without FA. Data were analyzed by one-way ANOVA with subsequent Bonferroni correction. Results revealed that calf hepatocytes treated with FA had greater content of non-esterified fatty acids (NEFA) and triacylglycerol (TAG), and greater mRNA and protein abundance of the lipid synthesis-related SREBF1 and FASN. In contrast, mRNA and protein of CPT1A (fatty acid oxidation) and the cholesterol metabolism-related targets SREBF2, HMGCR, ACAT2, APOA1, ABCA1 and ABCG5 was lower. Content of the antioxidant-related glutathione (GSH) and activities of superoxide dismutase (SOD) also was lower. Compared with FA challenge alone, 50 mu M I3 -sitosterol led to greater mRNA and protein abundance of SREBF2, HMGCR, ACAT2 and ABCG5, and greater content of GSH and activity of SOD. In contrast, compared with the FA group, the mRNA and protein abundance of SREBF1 and ACC1 and the content of TAG and NEFA in the I3 -sitosterol + FA group were lower. Overall, I3 -sitosterol can promote cholesterol metabolism and reduce oxidative stress while reducing lipid accumulation in hepatocytes challenged with high concentrations of fatty acids.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：黑龙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台