文献类型： 外文期刊

作者： Zhang, Qiu Qin ¹ ; Shi, Jie ¹ ; Shen, Pei Yao ¹ ; Xi, Fei ¹ ; Qian, Cheng Yu ¹ ; Zhang, Guo Hua ² ; Zhu, Hai Jun ³ ; Xiao, Hong Mei ¹ ;

作者机构： 1.Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China

2.Shandong Wukangxuan Modern Agr & Forestry Dev Co, Zoucheng 273519, Peoples R China

3.Jiangsu Acad Agr Sci, Nanjing 210014, Peoples R China

关键词： walnut; Botryosphaeria dothidea; postharvest disease; biocontrol agents; Bacillus amyloliquefaciens; Hanseniaspora uvarum

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2022 年 11 卷 22 期

页码：

收录情况： SCI

摘要： Biological control by antagonistic microorganisms are an effective and environmentally friendly approach in postharvest disease management. In order to develop a biocontrol agent for fresh walnut fruit preservation, the potential biocontrol effects of Bacillus amyloliquefaciens RD.006 and Hanseniaspora uvarum FA.006 against the main fungal pathogen of walnuts were evaluated. Botryosphaeria species showed the highest detection, and the INHT01 strain showed the strongest pathogenicity. Bot. dothidea INHT01 caused gray mold and brown rot on fresh walnuts, and its incidence rate reached 100% after an 8 days incubation. The growth of this fungal strain can be promoted by lighting, with a maximum growth rate achieved at a pH of 7 and at 28 degrees C. B. amyloliquefaciens RD.006 and H. uvarum FA.006 supernatants at a concentration of 1-15% v/v showed antifungal activity. The mycelial growth inhibition rates of Bot. dothidea INHT01 were 23.67-82.61% for B. amyloliquefaciens RD.006 and 1.45-21.74% for H. uvarum FA.006. During Bot. dothidea INHT01 growth, the biomass, nucleic acid leakage, and malondialdehyde content gradually increased, while the DPPH scavenging capacity and SOD activity decreased. The B. amyloliquefaciens RD.006 and H. uvarum FA.006 strains showed antifungal activity by damaging fungal cell membranes and reducing fungal antioxidant activity. Moreover, the antifungal effect of B. amyloliquefaciens RD.006 was higher than that of H. uvarum FA.006. Hence, the RD.006 strain of B. amyloliquefaciens can be considered a potential biocontrol agent for the management of postharvest walnut diseases caused by Bot. dothidea.