Integrated gill transcriptome and biochemical indices analyses reveal that acute salinity stress induces oxidative stress and immune and metabolic disorders in Red Tilapia (Oreochromis spp.)
文献类型： 外文期刊
作者： Huang, Renshan 1 ; Tao, Yifan 2 ; Jiang, Bingjie 2 ; Badran, Mohamed Fekri 3 ; Zhu, Jian 1 ; Hua, Jixiang 1 ; Wang, Qingchun 1 ; Lu, Siqi 2 ; Saleh, Moustafa Hassan Lotfy 3 ; Aboueleila, Rahma Halim Mahmoud 3 ; Xu, Pao 1 ; Qiang, Jun 1 ;
作者机构： 1.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214128, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resources, Minist Agr, Wuxi 214081, Peoples R China
3.Suez Canal Univ, Fish Farming & Technol Inst, Aquat Hatchery Prod Dept, Ismailia, Egypt
关键词： Salinity stress; Red tilapia; Gill; Metabolism; Transcriptome
期刊名称：AQUACULTURE （ 影响因子：3.9； 五年影响因子：4.4 ）
ISSN： 0044-8486
年卷期： 2025 年 599 卷
页码：
收录情况： SCI
摘要： Freshwater resources are increasingly strained in China, so fish that can tolerate saline water are favored for aquaculture. In this study, we explored the responses of the gills of red tilapia ( Oreochromis spp.) to salinity stress. First, the salinity tolerance of red tilapia was determined in an acute salinity toxicity assay. Then, red tilapia were cultured in fresh water (salinity: 0.0 %o, Ctrl group) or saline water (salinity: 20.3 +/- 0.1 %o, Salt group) for 24 h, and sampled at various time points to examine gill structure, physiological and biochemical indices, and the gill transcriptome. Saline water damaged gill tissues, causing shortening and thickening of gill vignettes and basal hyperplasia, and increasing the apoptosis rate. Saline water activated metabolism and the immune system, but impaired the antioxidant system. Transcriptome analyses revealed 3292 (1403 up-regulated and 1889 down- regulated) differentially expressed genes between the Salt and Ctrl groups at 24 h. Enrichment analyses revealed key pathways (beta-alanine metabolism, glycolysis /gluconeogenesis, theToll-like receptor signaling pathway, and the PPAR signaling pathway) that were activated in the gills of red tilapia under salinity stress. These pathways are mainly involved in osmotic pressure regulation, oxidative stress, energy metabolism, and the immune response. In summary, salinity damages red tilapia gills and causes oxidative stress; and up-regulation of genes involved in osmotic pressure regulation, metabolism, and immunity allows it to tolerate saline environments. Our results provide insights into the response mechanisms of red tilapia to salinity stress, and will be useful for the development of salt-tolerant red tilapia strains.
- 相关文献
作者其他论文 更多>>
-
Integration of transcriptome, histopathology, and physiological indicators reveals regulatory mechanisms of largemouth bass ( Micropterus salmoides) in response to carbonate alkalinity stress
作者：Hua, Jixiang;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Li, Yan;Dong, Yalun;Jiang, Bingjie;Xi, Bingwen;Qiang, Jun
关键词：Micropterus salmoides; Carbonate alkalinity stress; Tissue damage; Serum biological chemistry; RNA-seq
-
Regulatory patterns of testis and ovary maturation in largemouth bass (Micropterus salmoides) revealed by transcriptome profiling
作者：Wang, Qingchun;Lu, Siqi;Hua, Jixiang;Tao, Yifan;Qiang, Jun;Wang, Qingchun;Zhuge, Yan;Chen, Wenhua;Duan, Xuejun
关键词：Micropterus salmoides; Transcriptome; Gonad maturation; Growth dimorphism; FISH
-
Biochemical indices, histological observations and transcriptome sequencing reveal the response mechanism of largemouth bass (Micropterus salmoides) to transport stress
作者：Duan, Xue Jun;Tao, Yi Fan;Hua, Jixiang;Qiang, Jun;Wang, Qing Chun
关键词：Micropterus salmoides; Transport stress; Intestinal tissue; Oxidative stress; Apoptosis; Transcriptome
-
Muscle quality enhancement of largemouth bass (Micropterus salmoides) by alkaline water cultured environment: A comprehensive evaluation of physical features, nutrient composition, and flavor characteristics
作者：Hua, Jixiang;Jiao, Xidong;Wang, Wen;Xi, Bingwen;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Dong, Yalun;Wang, Wen;Xi, Bingwen;Qiang, Jun;Jiao, Xidong;Fan, Daming
关键词：Micropterus salmoides; Alkaline water culture; Muscle quality; Nutrient composition; GC-IMS; Volatile compounds
-
Gut Microbiota Contribute to Heterosis for Growth Trait and Muscle Nutrient Composition in Hybrid Largemouth Bass (Micropterus salmoides)
作者：Hua, Jixiang;Tao, Yifan;Sun, Hui;Liu, Kai;He, Jie;Qiang, Jun;Wang, Qingchun;Zhuge, Yan;Chen, Wenhua;Tao, Yifan;Lu, Siqi;Liu, Kai;Qiang, Jun
关键词：hybridization; microbiota taxa; amino acid; fatty acid; non-additive
-
Genetic Structure, Selective Signatures, and Single Nucleotide Polymorphism Fingerprints of Blue Tilapia (Oreochromis aureus), Nile Tilapia Oreochromis niloticus), and Red Tilapia (Oreochromis spp.), as Determined by Whole-Genome Resequencing
作者：Hua, Jixiang;Tao, Yifan;Wang, Qingchun;Sun, Hui;Qiang, Jun;Hua, Jixiang;Tao, Yifan;Lu, Siqi;Dong, Yalun;Qiang, Jun
关键词：tilapia; whole-genome resequencing; genetic diversity and structure; selective features; fingerprinting
-
Artificial intelligence in smart seafood safety across the supply chains: Recent advances and future prospects
作者：Jiao, Xidong;Zhu, Jinlin;Ye, Weijian;Zou, Hao;Yan, Bowen;Zhang, Nana;Zhang, Hao;Fan, Daming;Zhu, Jinlin;Ye, Weijian;Zou, Hao;Yan, Bowen;Zhang, Nana;Zhang, Hao;Jiao, Xidong;Qiang, Jun;Tao, Yifan;Jiao, Xidong;Qiang, Jun;Tao, Yifan;Zhang, Dachuan;Zhu, Jinlin;Fan, Daming;Fan, Daming
关键词：Artificial intelligence; Seafood safety; Aquaculture; Freshness monitoring; Seafood adulteration
