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Performance of DAMBO-P-H as a High Sensitive Fluorescence Indicator for Nitric Oxide

文献类型: 外文期刊

作者: Yang, Q. 1 ; Zhang, X. 2 ; Zhang, W. 2 ; Jiao, Y. 4 ; Jiao, B. 1 ; Wang, S. 4 ;

作者机构: 1.Second Mil Med Univ, Coll Basic Med Sci, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China

2.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China

3.Shanghai Ocean Univ, Coll Food Sci, Shanghai 201306, Peoples R China

4.Huaihai Inst Technol, Sch Marine, Lianyungang 222005, Peoples R China

期刊名称:PROCEEDINGS OF THE XIV BIENNIAL MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL

ISSN:

年卷期: 2008 年

页码:

收录情况: SCI

摘要: An emergent approach to the detection of nitric oxide (NO) in tissues relies on the use of fluorescence probes that are activated by products of NO autoxidation. Here we explore the performance of the pH-independent, highly selective and sensitive fluorescence NO indicator 8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4- difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (DAMBO-P-H)for the localization of sources of NO in human umbilical vein endothelial cell line, either from endogenous NO synthesis or from chemically released NO from targets of nitrosation/nitrosylation, and explore the specificity of DAMBO-P-H for NO in detail for its application to the biological systems.

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