Monitoring Nitric Oxide Release in Single Cells by Capillary Electrophoresis with Laser-induced Fluorescence Detection
文献类型： 外文期刊
作者： Zhang, X. 1 ; Yang, Q. 2 ; Zhang, W. 2 ; Hui, Y. 1 ; Jiang, P. 2 ; Yu, H. 1 ; Cai, Y. 1 ; Jiao, B. 2 ;
作者机构： 1.Chinese Acad Fishery Sci, East China Sea Fisheries Res Inst, Shanghai 200090, Peoples R China
2.Second Mil Med Univ, Dept Biochem & Mol Biol, Coll Basic Med Sci, Shanghai 200433, Peoples R China
期刊名称：PROCEEDINGS OF THE XIV BIENNIAL MEETING OF THE SOCIETY FOR FREE RADICAL RESEARCH INTERNATIONAL
ISSN：
年卷期： 2008 年
页码：
收录情况： SCI
摘要： Monitoring nitric oxide (NO) release at single cell level is fundamental to understand the diverse physiological/pathophysiological functions of this remarkable signal molecule. Here, a new versatile and selective analytical method using capillary electrophoresis with laser-induced fluorescence detection was originally developed for monitoring NO release in individual cells after choosing 8-(3,4-diaminophenyl)-2,6-bis(2-carboxyethyl)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (DAMBO-P-H). The high resolution quality of this method permit to quantify NO release in single cells quickly, reproducibly and selectively, yielding a sensitive approach to in situ single-cell analysis with a detection limit reaching attomole level. The feasibility of the method was fully validated by successfully applied to measurement of NO release in four single cell study models for the understanding of the pathobiological and therapeutic basis of NO for cardiovascular diseases, and for the study of the virus infection of high pathogenicity avian influenza virus (H5N1), HBV, HCV and human influenza virus A, respectively.
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