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Genetic differentiation among common and selected hatchery populations of founder: Evidence from RAPD markers

文献类型： 外文期刊

作者： Liu, Yun-Guo 1 ; Chen, Song-Lin 2 ; Li, Ba-Fang 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Ocean Univ China, Div Life Sci & Technol, Qingdao 266003, Peoples R China

关键词： flounder;Paralichthys olivaceus;RAPD marker;genetic differentiation

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

ISSN： 0305-1978

年卷期： 2007 年 35 卷 10 期

页码：

收录情况： SCI

摘要： Randomly amplified polymorphism DNA (RAPD) analysis was performed in order to evaluate one common population and two selected hatchery populations of flounder Paralichthys olivaceus. A total of 90 individuals belonging to three populations were screened using 12 different RAPD primers. A total of 71 loci were produced in the three studied populations, and 43.5%, 42.4% and 42.2% of these loci were polymorphic over all the genotypes tested in common, susceptible and resistant populations, respectively. The number of loci detected by single primers ranged from four to eight. The average heterozygosites of common, susceptible and resistant populations were 0.112, 0.103 and 0.111, respectively. Compared with the common population, the two selected hatchery populations, susceptible and resistant, showed some genetic differences including a smaller number of loci, a smaller number of polymorphic loci, a smaller number of genotypes and a smaller Shannon index. The present study confirmed that it is important to detect the genetic variability of the selected hatchery population for the conservation of natural flounder resources. (C) 2007 Elsevier Ltd. All rights reserved.

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