Integrating GWAS, linkage mapping and gene expression analyses reveal the genetic control of first branch height in Brassica napus L
文献类型： 外文期刊
作者： Dong, Zhixue 1 ; Tang, Minqiang 2 ; Cui, Xiaobo 2 ; Zhao, Chuanji 2 ; Tong, Chaobo 2 ; Liu, Yueying 2 ; Xiang, Yang 4 ; Li, Zaiyun 1 ; Huang, Junyan 2 ; Cheng, Xiaohui 2 ; Liu, Shengyi 2 ;
作者机构： 1.Huazhong Agr Univ, Coll Plant Sci & Technol, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs Peoples Republ China PR, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Wuhan, Peoples R China
3.Hainan Univ, Sch Forestry, Key Lab Genet & Germplasm Innovat Trop Special For, Minist Educ, Haikou, Peoples R China
4.Guizhou Acad Agr Sci, Guizhou Rapeseed Inst, Guiyang, Peoples R China
关键词： Brassica napus; first branch height; RIL; GWAS; TCP1
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Rapeseed (Brassica napus L.) is a crucial oil crop cultivated worldwide. First branch height, an essential component of rapeseed plant architecture, has an important effect on yield and mechanized harvesting; however, the underlying genetic mechanism remains unclear. In this study, based on the 60K single nucleotide polymorphism array and a recombinant inbred lines population derived from M083 and 888-5, a total of 19 QTLs were detected in five environments, distributed on linkage groups A02, A09, A10, C06, and C07, which explained phenotypic variation ranging from 4.87 to 29.87%. Furthermore, 26 significant SNPs were discovered on Chr.A02 by genome-wide association study in a diversity panel of 324 re-sequencing accessions. The major QTL of the first branch height trait was co-located on Chr.A02 by integrating linkage mapping and association mapping. Eleven candidate genes were screened via allelic variation analysis, inter-subgenomic synteny analysis, and differential expression of genes in parental shoot apical meristem tissues. Among these genes, BnaA02g13010D, which encodes a TCP transcription factor, was confirmed as the target gene according to gene function annotation, haplotype analysis, and full-length gene sequencing, which revealed that TATA insertion/deletion in the promoter region was closely linked to significantly phenotypic differences BnaA02.TCP1(M083) overexpression resulted in decreased branch height and increased branch number in Arabidopsis. These results provide a genetic basis for first branch height and the ideal architecture of B. napus.
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