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Silencing of Sly-miR160a enhanced postharvest chilling tolerance of tomato fruit

文献类型: 外文期刊

作者: Ni, Shen 1 ; Duan, Wenhui 1 ; Ye, Xiaoyun 1 ; Zhang, Zhengke 1 ; Meng, Lanhuan 1 ; Yang, Jiali 1 ; Zhu, Lisha 1 ; Wang, Qing 2 ; Song, Hongmiao 1 ; Xu, Xiangbin 1 ;

作者机构: 1.Hainan Univ, Sch Food Sci & Engn, Key Lab Food Nutr & Funct Food Hainan Prov, Haikou 570228, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Agrifood Proc & Nutr IAPN, Key Lab Vegetable Postharvest Treatment Minist Agr, Beijing Key Lab Fruit & Vegetable Storage & Proc, Beijing 100097, Peoples R China

关键词: MicroRNA160a; ARF17; Chilling injury; ABA

期刊名称:POSTHARVEST BIOLOGY AND TECHNOLOGY ( 影响因子:6.8; 五年影响因子:7.5 )

ISSN: 0925-5214

年卷期: 2025 年 227 卷

页码:

收录情况: SCI

摘要: Tomato fruit is usually stored at low temperature to maintain quality and extend shelf life, but it is sensitive to cold conditions and prone to chilling injury (CI) symptoms. In this study, the effect of Sly-miR160a on the postharvest cold tolerance of tomato fruit was investigated. The results showed that silencing of miR160a by a short tandem target mimic (miR160a-STTM) resulted in a decrease in fruit CI index and weight loss and an increase in fruit firmness compared with wild-type (WT) and tomato fruit overexpressing Sly-miR160a (miR160a-OE). Additionally, the levels of hydrogen peroxide (H2O2), superoxide anion (O2 center dot-), malondialdehyde (MDA), and electrolyte leakage (EL) in the fruit of miR160a-STTM decreased, while the content of total phenol increased. At low temperature, silencing of miR160a increased the expression of WRKY33 by up-regulating the target gene ARF17 in response to chilling injury stress. Meanwhile, miR160a-STTM fruit had higher ABA content and higher expression of cold-responsive genes such as ICE1a, CBF1, and COR. These findings suggest that the silencing of Sly-miR160a enhanced the postharvest cold tolerance of tomato fruit by reducing reactive oxygen species (ROS) accumulation, enhancing antioxidant capacity, ABA signaling pathway, and ICE1-CBF1-COR cold signaling pathway.

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