文献类型： 外文期刊

作者： Zhang, Zhike ¹ ;

作者机构： 1.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan, Peoples R China

关键词： expression; F. occidentalis; odorant-binding proteins; sequence analysis; structure prediction

期刊名称：BULLETIN OF ENTOMOLOGICAL RESEARCH （ 影响因子：1.6； 五年影响因子：1.7 ）

ISSN： 0007-4853

年卷期： 2025 年 115 卷 1 期

页码：

收录情况： SCI

摘要： Insect odorant-binding proteins (OBPs) are the key proteins in insect olfactory perception and play an important role in the perception and discrimination of insects. Frankliniella occidentalis is a polyphagous pest and seriously harms the quality and yield of fruits, flowers and crops worldwide. Therefore, the discovery of OBPs has greatly improved the understanding of behavioural response that mediates the chemoreception of F. occidentalis. To identify the OBP gene of F. occidentalis and its sequence and expression, rapid amplification cDNA ends (RACE) and qRT-PCR reaction system were performed. The results showed that the sequence of FoccOBP9 gene was 846 bp and the reading frame was 558 bp, encoding 185 amino acid residues, a 3 ' non-coding region of 195 bp and a 5 ' non-coding region of 93 bp.The molecular weight of the protein was about 20.08 kDa, and the isoelectric point was 8.89. FoccOBP9 was similar to AtumGOBP and CnipOBP2 (30%), followed by BdorGOBP, DficGOBP, DsuzGOBP, AalbOBP38, CmarOBP6 and SexiOBP. Phylogenetic analysis of the FoccOBP9 demonstrated that the FoccOBP9 had a relatively close evolutionary relationship with SgreOBP1, AtumGOBP, HeleOBP3, CbowOBP17, CnipOBP2 and CpalOBP2. The prediction of secondary structure showed that FoccOBP9 protein contained 135 amino acid residues forming alpha-helix, 91 amino acid residues forming beta-sheets and 24 amino acid residues forming beta-turning. However, three-dimensional structure prediction showed that the FoccOBP9 protein skeleton was composed of six alpha-helices and the loops connecting these helices. Dynamic observation of the three-dimensional structure revealed that five alpha-helices (alpha 1, alpha 2, alpha 4, alpha 5, alpha 6) were found in the structure. The expression profiles analysis revealed that FoccOBP9 are highly abundant in antenna significantly, followed by the head and belly, and almost no expression in the chest and foot. Therefore, the identification and analysis of OBP may be useful for monitoring and limiting the damage of F. occidentalis.