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Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China

文献类型： 外文期刊

作者： Wang, Jing 1 ; Hou, Lei 2 ; Wei, Li 1 ; Yan, Xv 1 ; Zhu, Shanshan 1 ; Quan, Rong 1 ; Li, Zixuan 1 ; Wang, Dan 1 ; Jiang, Haijun 1 ; Song, Jiangwei 1 ; Cui, Yongqiu 1 ; Liu, Jue 2 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing Key Lab Prevent & Control Infect Dis Lives, Beijing, Peoples R China

2.Yangzhou Univ, Coll Vet Med, Yangzhou, Peoples R China

3.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China

关键词： avian metapneumovirus subgroup C (aMPV; C); isolation and characterization; respiratory disease; nucleoprotein gene sequencing; pathogenicity

期刊名称：POULTRY SCIENCE （ 影响因子：4.014； 五年影响因子：4.192 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 1 期

页码：

收录情况： SCI

摘要： Avian metapneumovirus (aMPV) is an important causative agent that causes acute respiratory disease and egg-dropping in chickens and turkeys. Here, we characterized an aMPV subgroup C (aMPV/C) from 320-day-old broiler breeder chickens with severe respiratory diseases in Beijing, China, as evidenced by RT-PCR typing and confirmation of the nucleoprotein (N) gene sequence. The N gene sequence of the aMPV/ C strain (designated BJ17) exhibited no deletions or insertions and possessed 94.6% to 99.6% identity to those of published aMPV/C isolates. The phylogenetic tree of the nucleotide sequences constructed using the neighbor-joining clustering method showed that the BJ17 strain formed one cluster with other aMPV/C viruses and formed one subcluster with published Chi-nese aMPV/C isolates regardless of Muscovy duck or chicken origins. Comparative analysis of the N proteins showed that a unique amino acid residue D at position 110 might be associated with regional distribution due to its occurrence in all the Chinese aMPV/C isolates only. Strain BJ17 was successfully isolated by cultured Vero cell passage and further inoculated in 3-wk-old spe-cific-pathogen-free chickens for the examination of path-ogenicity. Animal experimental results showed that BJ17-inoculated chickens had severe respiratory diseases and inflammatory lesions, as demonstrated by patholog-ical changes and aMPV antigen in the nasal turbinate, tracheae, and lung tissues. These results enrich the avail-able information regarding the epidemiology and patho-genicity of aMPV/C in chickens, which may facilitate the development of effective measures against aMPV/C infection in China.

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