您好，欢迎访问吉林省农业科学院 机构知识库！
吉林省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Optimization of Callus Induction Conditions from Immature Embryos of Maizeunder Stress

文献类型： 外文期刊

作者： Wei, Xiaotong 1 ; Fan, Xuhong 2 ; Lu, Xuan 1 ; Han, Liyuan 1 ; Zhang, Honglin 3 ; Liu, Siyan 3 ; Qu, Jing 3 ; Guan, Shuyan 3 ; Ma, Yiyong 3 ;

作者机构： 1.Jilin Agr Univ, Coll Life Sci, Changchun 130118, Peoples R China

2.Jilin Acad Agr Sci, Changchun 130118, Peoples R China

3.Jilin Agr Univ, Joint Lab Int Cooperat Modem Agr Technol, Minist Educ, Changchun 130118, Peoples R China

关键词： Maize; immature embryo; adversity stress; medium optimization

期刊名称：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：1.407； 五年影响因子：0.996 ）

ISSN： 0031-9457

年卷期： 2022 年 91 卷 9 期

页码：

收录情况： SCI

摘要： The embryos of maize (ZeamaysL.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor mate-rials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000,mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Ortho-gonal test analysis showed that the best combination of medium components in induction was A2B3C1D3(2),namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mgmiddotmL-1, the concentration of L-Pro-line (L-Pro) was 0.8 mgmiddotmL-1, and the concentration of silver nitrate (AgNO3) was 10 mgmiddotmL-1(or 5 mgmiddotmL).Interestingly, we found that the optimal medium supplemented with 30 gmiddotL-1PEG6000or80gmiddotL-1mannitolwas suitable for antioxidant enzyme activity and malondialdehyde (MDA) content in GS07 callus. Exogenous10 mmolmiddotL-1Ca2+in the medium components with 100 mmolmiddotL-1sodium chloride (NaCl) could improve theactivity of antioxidant enzymes in GS07 callus. Callus of GS07 could divide normally and grow well in mediumcomponents with 27 mgmiddotL-1KH2PO4. This study enhanced the adaptability of maize callus to stress and optimizedthe culture conditions.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：吉林省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台