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Chitosan-platinum nanozyme-mediated ratiometric fluorescent immunosensor with dual catalytic cascades for sensitive detection of fenitrothion

文献类型： 外文期刊

作者： Liu, Jie 1 ; Deng, Hao 1 ; Yin, Qing-Chun 4 ; Wu, Min-Fu 5 ; Luo, Lin 3 ; Wang, Hong 3 ; Xu, Zhen-Lin 3 ; Jia, Bao-Zhu 2 ;

作者机构： 1.Hainan Acad Agr Sci, Inst Agroprod Proc & Design, Key Lab Trop Fruit & Vegetable Cold chain Hainan P, Haikou 571100, Peoples R China

2.Guangdong Univ Educ, Coll Biol & Food Engn, Guangzhou 510303, Peoples R China

3.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Peoples R China

4.Hainan Inst Food Control, Key Lab Trop Fruits & Vegetables Qual & Safety Sta, Haikou 570314, Peoples R China

5.Foshan Polytech, Dept Food Sci, Foshan 528137, Peoples R China

关键词： Nanozyme; Catalytic cascades; Ratiometric fluorescence; Immunosensor

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2025 年 293 卷

页码：

收录情况： SCI

摘要： Organophosphorus pesticide residues pose significant threats to food safety and environmental health, and their monitoring methods demand sensitivity and anti-interference capability. In this study, we developed a novel ratiometric fluorescent immunosensor for fenitrothion (FN) detection by employing chitosan-modified platinum nanoparticles (Ch-Pt NPs) as catalytic nanozymes. Ch-Pt NPs exhibit oxidase-mimicking activity, enabling the oxidation of ascorbic acid (AA) and o-phenylenediamine (OPD) to produce dehydroascorbic acid (DHAA) and 2,3-diaminophenazine (DAP, Em = 568 nm), respectively. Then, DHAA further reacted with the remaining OPD to generate quinoxaline derivatives (DFQ, Em = 430 nm). These two products further form ratiometric fluorescent signals. Taking AA as a breakthrough point, the dual-catalytic cascade of natural enzymes and nanozymes was achieved by the combination with the alkaline phosphatase (ALP) -based enzyme-linked immunosorbent assay (ALP-ELISA). Compared with the conventional colorimetric ELISA, the proposed sensor demonstrated a nearly 20-fold enhancement in sensitivity (detection limit: 0.48 ng/mL) and achieved satisfactory recoveries of 80.0-108.3 % in spiked samples. The ratiometric fluorescent immunosensor demonstrates outstanding performance and holds great potential for extensive applications in the field of pesticide monitoring.

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