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Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways

文献类型： 外文期刊

作者： Luo, Shengyu 1 ; Liu, Cheng 1 ; Gao, Xinming 1 ; Wang, Jingqian 1 ; Zhang, Yibo 1 ; Ding, Jie 1 ; Hou, Congcong 1 ; Zhu, Junquan 1 ; Lou, Bao 2 ; Shen, Weiliang 3 ; Wu, Xiongfei 3 ; Zhang, Chundan 1 ;

作者机构： 1.Ningbo Univ, Sch Marine Sci, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Peoples R China

2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

3.Ningbo Acad Oceanol & Fishery, State Key Lab Large Yellow Croaker Breeding, Ningbo 315103, Peoples R China

关键词： hypoxia; large yellow croaker; mitochondrial apoptosis pathway; death receptor apoptosis pathway

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.6； 五年影响因子：1.6 ）

ISSN： 2096-5508

年卷期： 2023 年 41 卷 6 期

页码：

收录情况： SCI

摘要： Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea, an economically important mariculture fish in China. Apoptosis is a consequence of hypoxia on fish. However, the effects of hypoxia stress on apoptosis in L. crocea remain largely unknown. We investigated the effect of environmental hypoxia on apoptosis in L. crocea. Results show that hypoxia induced apoptosis in L. crocea both in vivo and in vitro. The mitochondrial membrane potential was significantly reduced in large yellow croaker fry (LYCF) cells. The expression levels of B-cell lymphoma/leukemia-2 (Bcl-2) mRNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress, respectively, whereas the expression level of Bcl-2 associated X (Bax) mRNA, Casp3 mRNA, and activity of caspase-3/7/9 were significantly increased, indicating that hypoxia induced caspase-dependent intrinsic apoptosis in L. crocea. The expression level of the apoptosis-inducing factor (AIF) protein was significantly increased in the liver and LYCF cells. The level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of L. crocea, demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic apoptosis. In addition, the activity of caspase-8 was significantly increased, indicating that hypoxia stress induced extrinsic apoptosis in L. crocea. Therefore, hypoxia induced apoptosis in L. crocea through both the intrinsic and extrinsic pathways. The present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

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