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Cloning and characterization of a rice field eel vasa-like gene cDNA and its expression in gonads during natural sex transformation

文献类型： 外文期刊

作者： Ye, Ding 1 ; Lv, Daoyuan 2 ; Song, Ping 2 ; Peng, Maoyu 2 ; Chen, Yungui 2 ; Guo, Ming 2 ; Yang, Qiwen 3 ; Hu, Yinchang;

作者机构： 1.Wuhan Univ, Coll Life Sci, Lab Mol Genet & Dev Biol, Wuhan 430072, Hubei, Peoples R China

2.Wuhan Univ, Coll Life Sci, Lab Mol Genet & Dev Biol, Wuhan 430072, Hubei, Peoples R China; Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China

3.Wuhan Univ, C

关键词： vasa;rice field eel;sex transformation

期刊名称：BIOCHEMICAL GENETICS （ 影响因子：1.89； 五年影响因子：1.959 ）

ISSN： 0006-2928

年卷期： 2007 年 45 卷 3-4 期

页码：

收录情况： SCI

摘要： The vasa (vas)-related gene encodes an RNA helicase protein member of the DEAD-box family and plays key roles in germ-cell formation in higher metazoans. Using degenerate PCR and RACE, we cloned the vasa gene of the rice field eel (Monopterus albus), which is homologous to the Drosophila vasa gene. We named it ma-vas (Monopterus albus vas). Ma-vas encodes a protein of 618 amino acids, which contains all of the known characteristics of vasa homologs. RT-PCR analysis revealed that ma-vas was exclusively expressed in the gonads of the female, intersex, and male. During gonadal natural sex reversal, ma-vas is expressed in oocytes at all stages of oogenesis, in degenerating oocytes of ovotestis, and in spermatogonia and spermatocytes at early stages. The vasa positive signal was also observed in the peripheral layer of late ovary. It was not found, however, in that layer of the testis. Alkaline phosphatase (AKP) staining on the ovary and testis also indicated that some cells had differentiational potential in the peripheral layer of the ovary, suggesting that spermatogonia might arise from cells with AKP and vasa-positive staining in the peripheral layer of the female gonad.

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