文献类型： 外文期刊
作者： Liu, L. 1 ; Wei, Q. 1 ; Guo, F. 1 ; Zhang, J. 1 ; Zhang, T. 1 ;
作者机构： 1.Chinese Acad Fisheries Sci, Yangtse River Fisheries Res Inst, Key Lab Freshwater Fish Germplasm Resources & Bio, Jinzhou 434000, Peoples R China
期刊名称：JOURNAL OF APPLIED ICHTHYOLOGY （ 影响因子：0.892； 五年影响因子：0.956 ）
ISSN： 0175-8659
年卷期： 2006 年 22 卷
页码：
收录情况： SCI
摘要： This investigation studied the effectiveness of techniques to collect sperm from Chinese sturgeon males and developed examination methods fon the viability of sperm. Further, a series of experiments tested a variety of sperm diluents while also selecting suitable cryoprotectants and its concentration, determined optimal equilibrium time, cooling rate and freezing point. Additionally, the effects of the cryo-preservation methods on the viability of sperm were tested while also determining the fertilization success with sperm cryo-preserved with three different diluents. The results indicated that the diluent D-5 (8.85g/L NaCl; 0.20g/L and 0.40g/L NaHCO3) was the best sperm diluent, and 12% DMSO was a suitable cryo-protectant keeping 0.5-2h as the equilibrium time at 4. Combining D-5 with DMSO under the described conditions seems to be the most suitable method for sturgeon sperm cyro-proservation while employing the cooling rate at 2/min and freezing point at -6 keeping the sperm for 10 min before placing into liquid nitrogen. Fertilization tests, D-3, D-5 and D-6, (whose effects were similar in the diluents tests), were used to compare the effects of cryopreserved sperm on fertilization success. The results indicated that the effect of D-5 in thawed sperm fertilization was the best among all three diluents having the least negative effect. The resulting in a fertilization rate was 83.8% and survival rate of larvae reached up to 68.1%. However, comparing with the control, the survival rate of D-5 was still noticeably smaller. The reason needs to further study.
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