Cryopreservation of the sperm of spotted halibut Verasper variegates (Pleuronectiformes, Pleuronectidae)
文献类型: 外文期刊
作者: Liu, XZ 1 ; Zhang, SC 2 ; Zhang, YZ 2 ; Xu, YJ 2 ;
作者机构: 1.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
2.Chinese Acad Fisheries Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China; Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China
关键词: cryopreservation;sperm;spotted halibut;Verasper variegate
期刊名称:INDIAN JOURNAL OF MARINE SCIENCES ( 影响因子:0.496; 五年影响因子:0.605 )
ISSN: 0379-5136
年卷期: 2006 年 35 卷 1 期
页码:
收录情况: SCI
摘要: The sperm of spotted halibut Verasper variegates was cryopreserved by a simple and convenient protocol. The cryoprotectants used were dimethyl sulfoxide (Me2SO), glycerol (Gly), methanol (MeOH), Me2SO + egg yolks, Gly + egg yolk and MeOH + egg yolk. The percentage of forward motile sperm in semen cryopreserved in the different extenders with Me2SO, Gly, MeOH, Me2SO + egg yolk, Gly + egg yolk and MeOH + egg yolk was 40.5 +/- 4.2%, 49.5 +/- 4.2%, 38.3 +/- 2.4%, 65.0 +/- 4.0%, 67.5 +/- 2.9% and 49.5 +/- 8.4%, respectively. The fertilization and hatching rates of cryopreserved sperm were from 59 +/- 2.4% to 70 +/- 3.0%, and from 11 +/- 3.0% to 18 +/- 2.6%, respectively. These tended to be highest when Gly + egg yolk or Me2SO + egg yolk were used as cryoprotectants. Under SEM, it could be seen that while the majority of the freeze-thawed sperm remained morphologically normal, some exhibited damaged or lost mitochondria, which possibly caused the decrease in motility and fertility of the freeze-thawed sperm.
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