文献类型： 外文期刊

作者： Wang, GG ¹ ; Lin, W ² ; Zhang, LJ ² ; Yan, XJ ² ; Duan, DL ² ;

作者机构： 1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

2.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

关键词： alginic acid decomposing bacterium;caspase-3;Laminaria japonica;hypersensitive reaction (HR);programmed cell death (PCD);TUNEL

期刊名称：PROGRESS IN NATURAL SCIENCE （ 影响因子：3.607； 五年影响因子：4.795 ）

ISSN： 1002-0071

年卷期： 2004 年 14 卷 12 期

页码：

收录情况： SCI

摘要： TdT-mediated dUTP-biotin nick end labeling (TUNEL) is a sensitive and valid method for detecting DNA cleavage in programmed cell death (PCD). Using this method, DNA cleavage was observed in Laminaria japonica sporophytic tissues, which were infected with alginic acid decomposing bacterium. It was found that DNA cleavage occurred 5 min after the infection, the fragments with 3'-OH groups of cleaved nuclear DNA increased with time of infection and spread from the infection site. Although no typical DNA ladder (200 bp/ 180 bp) was detected by routine agarose gel electrophoresis, the cleavage of nuclear DNA fragments of 97 similar to 48.5 kb could be detected by pulsed field gel electrophoresis (PFGE). By using CaspGLOW(TM) fluorescein active caspase-3 staining method, caspase-3 activity has been detected in response to the infection of alginic acid decomposing bacterium. Our results are similar to the observations in hypersensitive response (HR) of higher plant, suggesting that the rapid cell death of L. japonica infected by alginic acid decomposing bacterium might be involved in PCD, and indicating that the occurrence of PCD is an active defense process against the pathogen's infection.