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Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion

文献类型: 外文期刊

作者: Hongtuo, F 1 ; Chingjiang, W 2 ;

作者机构: 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Jiangsu, Peoples R China

2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

关键词: electrofusion;nuclear transfer;UV inactivation;fish;oocyte;blastomere;gastrula cell

期刊名称:AQUACULTURE RESEARCH ( 影响因子:2.082; 五年影响因子:2.415 )

ISSN: 1355-557X

年卷期: 2001 年 32 卷 4 期

页码:

收录情况: SCI

摘要: To study nuclear transfer in the leach (Paramisgurnus dabryanus Sauvage), blastula and gastrula cells were fused with UV-inactivated oocytes by cell-to-cell electrofusion. To facilitate nuclear transfer, blastula and gastrula cells were cultured or incubated at 4 degreesC in different solutions. TC-199 medium supplemented with 20% calf serum was the best culture solution, and effectively retained the totipotence of blastula or gastrula cells for up to 10 days, It was found that gastrula cells incubated at 4 degreesC had the same totipotence as blastula cells, The optimal UV dosage for inactivation of the oocyte chromatin was 180-240 mJ cm(-2). Electrofusion was carried out in a cone-shaped fusion chamber, which permitted the recipient oocyte and the donor blastula cell to contact one another. The electrofusion procedure resulted in a 10% success rate of normal-appearing fish. Genetic analysis indicated that the nuclear material originated from the donor cell (blastomere) and the oocyte pronucleus did not take part in development.

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