您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Functional characterization of rice (Oryza sativa) thioredoxins for detoxification and degradation of atrazine

文献类型： 外文期刊

作者： Ma, Li Ya 1 ; Lu, Yingfei 1 ; Cheng, Jinjin 1 ; Wan, Qun 1 ; Ge, Jing 1 ; Wang, Ya 1 ; Li, Yong 1 ; Feng, Fayun 1 ; Li, Mei 1 ; Yu, Xiangyang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Zhongling St 50, Nanjing 210014, Peoples R China

2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Zhongling St 50, Nanjing 210014, Peoples R China

3.Anhui Agr Univ, Coll Resources & Environm, Hefei 230036, Peoples R China

关键词： Thioredoxins; Atrazine; Transcriptome; Rice; Degradation and detoxification

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 877 卷

页码：

收录情况： SCI

摘要： Thioredoxins (TRXs) are a group of antioxidant enzymes that play a critical role in plant growth and resistance to stress. However, the functional role and mechanism of rice TRXs in response to pesticides (e.g. atrazine, ATZ) stress remain largely unexplored. Here, 24 differentially expressed TRX genes (14 up and 10 down) of ATZ-exposed rice were identified through high-throughput RNA-sequencing analysis. Twenty-four TRX genes were unevenly mapped to 11 chromosomes and some of the genes were validated by quantitative RT-PCR. Bioin-formatics analysis revealed that ATZ-responsive TRX genes contain multiple functional cis-elements and conserved domains. To demonstrate the functional role of the genes in ATZ degradation, one representative TRX gene LOC_Os07g08840 was transformed into yeast cells and observed significantly lower ATZ content compared to the control. Using LC-Q-TOF-MS/MS, five metabolites were characterized. One hydroxylation (HA) and two N- dealkylation products (DIA and DEA) were significantly increased in the medium with positive transformants. Our work indicated that TRX-coding genes here were responsible for ATZ degradation, suggesting that thio-redoxins could be one of the vital strategies for pesticide degradation and detoxification in crops.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台