Functional characterization of rice (Oryza sativa) thioredoxins for detoxification and degradation of atrazine
文献类型： 外文期刊
作者： Ma, Li Ya 1 ; Lu, Yingfei 1 ; Cheng, Jinjin 1 ; Wan, Qun 1 ; Ge, Jing 1 ; Wang, Ya 1 ; Li, Yong 1 ; Feng, Fayun 1 ; Li, Mei 1 ; Yu, Xiangyang 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Zhongling St 50, Nanjing 210014, Peoples R China
2.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Zhongling St 50, Nanjing 210014, Peoples R China
3.Anhui Agr Univ, Coll Resources & Environm, Hefei 230036, Peoples R China
关键词： Thioredoxins; Atrazine; Transcriptome; Rice; Degradation and detoxification
期刊名称：GENE （ 2022影响因子：3.5； 五年影响因子：3.3 ）
ISSN： 0378-1119
年卷期： 2023 年 877 卷
收录情况： SCI
摘要： Thioredoxins (TRXs) are a group of antioxidant enzymes that play a critical role in plant growth and resistance to stress. However, the functional role and mechanism of rice TRXs in response to pesticides (e.g. atrazine, ATZ) stress remain largely unexplored. Here, 24 differentially expressed TRX genes (14 up and 10 down) of ATZ-exposed rice were identified through high-throughput RNA-sequencing analysis. Twenty-four TRX genes were unevenly mapped to 11 chromosomes and some of the genes were validated by quantitative RT-PCR. Bioin-formatics analysis revealed that ATZ-responsive TRX genes contain multiple functional cis-elements and conserved domains. To demonstrate the functional role of the genes in ATZ degradation, one representative TRX gene LOC_Os07g08840 was transformed into yeast cells and observed significantly lower ATZ content compared to the control. Using LC-Q-TOF-MS/MS, five metabolites were characterized. One hydroxylation (HA) and two N- dealkylation products (DIA and DEA) were significantly increased in the medium with positive transformants. Our work indicated that TRX-coding genes here were responsible for ATZ degradation, suggesting that thio-redoxins could be one of the vital strategies for pesticide degradation and detoxification in crops.
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