Ribosome Profiling and RNA Sequencing Reveal Genome-Wide Cellular Translation and Transcription Regulation Under Osmotic Stress in Lactobacillus rhamnosus ATCC 53103
文献类型： 外文期刊
作者： Fan, Xuejing 1 ; Bao, Tianyu 1 ; Yi, Huaxi 2 ; Zhang, Zongcai 1 ; Zhang, Kenan 1 ; Liu, Xin 1 ; Lin, Xue 1 ; Zhang, Zhen 1 ; Feng, Zhen 1 ;
作者机构： 1.Northeast Agr Univ, Coll Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, Qingdao, Peoples R China
3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning, Peoples R China
关键词： L; rhamnosus; ribosome profiling; osmotic stress; translation regulation; translation efficiency
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： To determine whether osmotic pressure affects the translation efficiency of Lactobacillus rhamnosus, the ribosome profiling assay was performed to analyze the changes in translation efficiency in L. rhamnosus ATCC 53103. Under osmotic stress, differentially expressed genes (DEGs) involved in fatty acid biosynthesis and metabolism, ribosome, and purine metabolism pathways were co-regulated with consistent expression direction at translation and transcription levels. DEGs involved in the biosynthesis of phenylalanine, tyrosine, and tryptophan, and the phosphotransferase system pathways also were co-regulated at translation and transcription levels, while they showed opposite expression direction at two levels. Moreover, DEGs involved in the two-component system, amino acid metabolism, and pyruvate metabolism pathways were only regulated at the transcription level. And DEGs involved in fructose and mannose metabolism were only regulated at the translation level. The translation efficiency of DEGs involved in the biosynthesis of amino acids was downregulated while in quorum sensing and PTS pathways was upregulated. In addition, the ribosome footprints accumulated in open reading frame regions resulted in impaired translation initiation and elongation under osmotic stress. In summary, L. rhamnosus ATCC 53103 could respond to osmotic stress by translation regulation and control the balance between survival and growth of cells by transcription and translation.
- 相关文献
作者其他论文 更多>>
-
Insight into the effects of different ripeness levels on the quality and flavor chemistry of Noni fruit ( Morinda citrifolia L.) . )
作者：Chen, Xiaoai;Gu, Chunhe;Zhu, Kexue;Xu, Fei;Feng, Zhen;Zhang, Yanjun;Chen, Xiaoai;Gu, Chunhe;Zhu, Kexue;Xu, Fei;Feng, Zhen;Zhang, Yanjun;Chen, Xiaoai;Gu, Chunhe;Zhu, Kexue;Xu, Fei;Feng, Zhen;Zhang, Yanjun;Chen, Xiaoai;Gu, Chunhe;Zhu, Kexue;Xu, Fei;Feng, Zhen;Zhang, Yanjun
关键词：Noni fruit; Quality and flavor; Physicochemical analysis; Electronic nose; Electronic tongue; GC-MS
-
Nutrient consumption patterns of Lactobacillus acidophilus
作者：Qiao, Yali;Wang, Mengrui;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Chen, Junxia;Liu, Fei;Yin, Boxing;Zhou, Wei;Feng, Zhen;Feng, Zhen;Feng, Zhen;Feng, Zhen;Liu, Fei
关键词：acid stress; exogenous nutrients; Lactobacillus acidophilus; nutrient consumption pattern
-
Unveiling the dynamic microbial succession and metabolic characteristics during traditional fermentation of hainan shanlan rice wine
作者：Guo, Xiaoxue;Wang, Mengrui;Zhou, Junping;Chen, Junxia;Chang, Ziqing;Yue, Mingzhe;Liu, Fei;Gu, Chunhe;Feng, Zhen
关键词：Hainan shanlan rice wine; Microbial diversity; Volatile metabolites; Non-volatile metabolites; Correlation analysis
-
Integrating shotgun metagenomics and metabolomics to elucidate the dynamics of microbial communities and metabolites in fine flavor cocoa fermentation in Hainan
作者：Chang, Haode;Wang, Mengrui;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Chen, Junxia;Gu, Chunhe;Qin, Xiaowei;Feng, Zhen;Qin, Xiaowei;Feng, Zhen
关键词：Cocoa fermentation; Shotgun metagenomics; Microbiota; Metabolome-microbiome interactions; Metabolic pathway
-
Effect of the initial pH of the culture medium on the nutrient consumption pattern of Bifidobacterium animalis subsp. lactis Bb12 and the improvement of acid resistance by purine and pyrimidine compounds
作者：Zhang, Zongcai;Liu, Fei;Wang, Mengrui;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Chen, Junxia;Yin, Boxing;Zhou, Wei;Feng, Zhen;Feng, Zhen
关键词：Bifidobacterium animalis subsp. lactis Bb12; acid stress; nutrient consumption pattern; acid resistance
-
Bioactivity and influence on colonic microbiota of polyphenols from noni (Morinda citrifolia L.) fruit under simulated gastrointestinal digestion
作者：Chen, Juanyun;Chen, Xiaoai;Zhang, Yanjun;Feng, Zhen;Zhu, Kexue;Xu, Fei;Gu, Chunhe;Chen, Juanyun;Chen, Xiaoai;Zhang, Yanjun;Feng, Zhen;Zhu, Kexue;Xu, Fei;Gu, Chunhe;Chen, Juanyun
关键词：Noni fruit; Ripeness level; Polyphenol; Antioxidant activity; Gut microbiome
-
PBAT microplastics exacerbates N2O emissions from tropical latosols mainly via stimulating denitrification
作者：Zhang, Zhen;Gao, Wenlong;Wu, Dongming;Zhang, Wen;Wang, Xiaotong;Liu, Huiran;Li, Qinfen;Fan, Changhua;Chen, Miao;Peng, Licheng;Wang, Xiaotong;Gao, Wenlong;Wu, Dongming;Zhang, Wen;Liu, Huiran;Li, Qinfen;Fan, Changhua;Chen, Miao;Gao, Wenlong;Wu, Dongming;Zhang, Wen;Liu, Huiran;Li, Qinfen;Fan, Changhua;Chen, Miao;Gao, Wenlong;Wu, Dongming;Zhang, Wen;Liu, Huiran;Li, Qinfen;Fan, Changhua;Chen, Miao
关键词：Biodegradable microplastics; Nitrous oxide emission; Nitrogen transformation; Microbial community; Tropical agricultural soil
