Ribosome Profiling and RNA Sequencing Reveal Genome-Wide Cellular Translation and Transcription Regulation Under Osmotic Stress in Lactobacillus rhamnosus ATCC 53103
文献类型： 外文期刊
作者： Fan, Xuejing 1 ; Bao, Tianyu 1 ; Yi, Huaxi 2 ; Zhang, Zongcai 1 ; Zhang, Kenan 1 ; Liu, Xin 1 ; Lin, Xue 1 ; Zhang, Zhen 1 ; Feng, Zhen 1 ;
作者机构： 1.Northeast Agr Univ, Coll Food Sci, Minist Educ, Key Lab Dairy Sci, Harbin, Peoples R China
2.Ocean Univ China, Coll Food Sci & Engn, Qingdao, Peoples R China
3.Chinese Acad Trop Agr Sci, Spice & Beverage Res Inst, Wanning, Peoples R China
关键词： L; rhamnosus; ribosome profiling; osmotic stress; translation regulation; translation efficiency
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）
ISSN：
年卷期： 2021 年 12 卷
页码：
收录情况： SCI
摘要： To determine whether osmotic pressure affects the translation efficiency of Lactobacillus rhamnosus, the ribosome profiling assay was performed to analyze the changes in translation efficiency in L. rhamnosus ATCC 53103. Under osmotic stress, differentially expressed genes (DEGs) involved in fatty acid biosynthesis and metabolism, ribosome, and purine metabolism pathways were co-regulated with consistent expression direction at translation and transcription levels. DEGs involved in the biosynthesis of phenylalanine, tyrosine, and tryptophan, and the phosphotransferase system pathways also were co-regulated at translation and transcription levels, while they showed opposite expression direction at two levels. Moreover, DEGs involved in the two-component system, amino acid metabolism, and pyruvate metabolism pathways were only regulated at the transcription level. And DEGs involved in fructose and mannose metabolism were only regulated at the translation level. The translation efficiency of DEGs involved in the biosynthesis of amino acids was downregulated while in quorum sensing and PTS pathways was upregulated. In addition, the ribosome footprints accumulated in open reading frame regions resulted in impaired translation initiation and elongation under osmotic stress. In summary, L. rhamnosus ATCC 53103 could respond to osmotic stress by translation regulation and control the balance between survival and growth of cells by transcription and translation.
- 相关文献
作者其他论文 更多>>
-
Effect of temperature regulation on microbial community, volatile flavours, amino acid profiles, and iridoid glycosides during noni (Morinda citrifolia L.) fruit fermentation
作者：Zhang, Chao;Gu, Chunhe;Wang, Mengrui;Chen, Junxia;Chang, Haode;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Zhang, Wenjing;Zhang, Quanmiao;Feng, Zhen;Zhang, Chao;Wang, Mengrui;Chen, Junxia;Chang, Haode;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Zhang, Wenjing;Zhang, Quanmiao;Feng, Zhen;Gu, Chunhe;Feng, Zhen
关键词：Shotgun metagenomics; Noni fruit; Volatile flavours; Amino acid profiles; Iridoid glycosides
-
Changes in key volatile components associated with leaf quality of Pandanus amaryllifolius Roxb. alongside growth duration
作者：Yan, Lu;Zhang, Ang;Qin, Xiaowei;Yu, Huan;Ji, Xunzhi;He, Shuzhen;Zong, Ying;Gu, Chunhe;Feng, Zhen;Hu, Lisong;Lu, Zhiqing;Yan, Lu;Lu, Zhiqing
关键词：GC-MS; VOCs; 2-Acetyl-1-pyrroline; Squalene; Agronomic traits; Leaf quality
-
Molecular mechanisms for exopolysaccharides synthesis in Lactobacillus helveticus: Relationship between structural characteristics and genomics
作者：Zhang, Kangyong;Duan, Sufang;Feng, Zhen;Li, Bailiang;Liu, Fei;Zhang, Xuemei;Qi, Xuefeng;Feng, Zhen;Gong, Pimin;Wu, Zhen
关键词：Exopolysaccharides; Lactobacillus helveticus; Structure; Characteristics; Genomics; Biosynthesis
-
Screening and characterizing indigenous yeasts, lactic acid bacteria, and acetic acid bacteria from cocoa fermentation in Hainan for aroma Development
作者：Chang, Haode;Gu, Chunhe;Feng, Zhen;Chang, Haode;Wang, Mengrui;Chen, Junxia;Yue, Mingzhe;Zhou, Junping;Chang, Ziqing;Zhang, Chao;Liu, Fei
关键词：cocoa; fermentation; flavor chemistry; starter cultures; yeasts
-
Investigation of aromatic compounds and olfactory profiles in cocoa pulp fermentation using yeast-based starters: A Volatilomics and machine learning approach
作者：Chang, Haode;Zhang, Quanmiao;Zhang, Wenjing;Liu, Fei;Gu, Chunhe;Ma, Liru;Feng, Zhen;Gu, Chunhe;Ma, Liru;Feng, Zhen;Ma, Liru
关键词：Cocoa; Fermentation; Machine learning; Aroma; GC-MS
-
Insight into peroxidase-mediated Morinda citrifolia L. (noni) fruit juice browning and precipitation, and a thermal inactivation strategy
作者：Zhang, Chao;Wang, Mengrui;Chen, Junxia;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Feng, Zhen;Zhang, Chao;Wang, Mengrui;Chen, Junxia;Chang, Ziqing;Zhou, Junping;Yue, Mingzhe;Gu, Chunhe;Feng, Zhen;Gu, Chunhe;Feng, Zhen
关键词：Morinda citrifolia L.; Peroxidase-mediated browning and; precipitation; Thermal inactivation
-
A novel framework-targeted affinity-based sandwich photoelectrochemical sensor for trace acrylamide determination
作者：Zhu, Wenqian;Cao, Wenwen;Yi, Zhongsheng;Li, Shuhuai;Ma, Xionghui;Li, Jianping;Chen, Rui;Zhang, Zhen;Pang, Chaohai;Li, Shuhuai;Ma, Xionghui
关键词：Photoelectrochemical sensor; Acrylamide; Covalent organic framework; Sandwich-type; Aptamer
