Enhanced O/W emulsifying properties of pea proteins via deamidation: Insights into interfacial behavior
文献类型： 外文期刊
作者： Luo, Lijuan 1 ; Li, Ping 1 ; Deng, Yuanyuan 1 ; Liu, Guang 1 ; Shi, Linfan 2 ; Zhang, Yan 1 ; Tang, Xiaojun 1 ; Zhou, Pengfei 1 ; Zhao, Zhihao 1 ; Zhang, Mingwei 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, Guangdong Key Lab Agr Prod Proc, Sericultural & Agrifood Res Inst,Key Lab Funct Foo, Guangzhou 510610, Peoples R China
2.Jimei Univ, Coll Ocean Food & Biol Engn, Xiamen 361021, Peoples R China
3.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China
关键词： Interfacial rheology; Large amplitude oscillatory dilatation; Emulsion stability
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 280 卷
页码：
收录情况： SCI
摘要： This study examines the effects of protein glutaminase modification on the interfacial properties and emulsion stability of pea protein isolates (PPI). Emulsions were prepared using native (NPPI) and deamidated PPI (DPPI) at concentrations from 0.5 wt% to 3.6 wt%. The stability of these emulsions was evaluated by examining droplet size distribution, flocculation index, zeta-potential, and CLSM. DPPI demonstrated superior emulsifying ability and stability, requiring only 2.0 wt% to prevent flocculation compared to NPPI's 3.6 wt%. Interfacial properties, such as protein coverage, composition, thickness, tension, and rheology, were characterized. Large Amplitude Oscillatory Dilatation analysis showed minimal differences between NPPI and DPPI-stabilized interfaces at 1 wt %. However, at 3.6 wt%, NPPI interfaces demonstrated abrupt intra-cycle yielding and viscous behavior, whereas DPPI interfaces exhibited gradual softening and a higher maximum linear strain. Additionally, DPPI showed higher interfacial protein coverage and lower interfacial tension. NPPI formed dense, brittle films prone to rupture under dynamic deformation, leading to poor stability. Deamidation of PPI unfolded the protein structure, exposing hydrophobic groups and increasing carboxyl groups, which reduced aggregation. This resulted in a uniform, extensible, and elastic interfacial film resistant to large deformations. Thus, DPPI-stabilized emulsions demonstrated superior stability, showcasing their potential for industrial applications.
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