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Genotyping and biofilm formation of Mycoplasma hyopneumoniae and their association with virulence

文献类型： 外文期刊

作者： Wu, Yuzi 1 ; Yu, Yanfei 1 ; Hua, Lizhong 1 ; Wei, Yanna 1 ; Gan, Yuan 1 ; Chenia, Hafizah Yousuf 4 ; Wang, Yixuan 5 ; Xie, Xing 1 ; Wang, Jia 1 ; Liu, Maojun 1 ; Shao, Guoqing 1 ; Xiong, Qiyan 1 ; Feng, Zhixin 1 ;

作者机构： 1.Minist Agr & Rural Affairs, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Jiangsu Acad Agr Sci, Nanjing, Peoples R China

2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing, Peoples R China

4.Univ KwaZulu Natal, Sch Life Sci, Discipline Microbiol, Durban, South Africa

5.Univ Warwick, Coventry CV4 7AL, W Midlands, England

6.Jiangsu Univ, Sch Life Sci, Zhenjiang, Jiangsu, Peoples R China

7.Jiangsu Vocat Coll Agr & Forestry, Sch Anim Husb & Vet Med, Jurong, Peoples R China

关键词： Mycoplasma hyopneumoniae; virulence; genotyping; biofilm

期刊名称：VETERINARY RESEARCH （ 影响因子：3.829； 五年影响因子：4.269 ）

ISSN： 0928-4249

年卷期： 2022 年 53 卷 1 期

页码：

收录情况： SCI

摘要： Mycoplasma hyopneumoniae, the causative agent of swine respiratory disease, demonstrates differences in virulence. However, factors associated with this variation remain unknown. We herein evaluated the association between differences in virulence and genotypes as well as phenotype (i.e., biofilm formation ability). Strains 168 L, RM48, XLW-2, and J show low virulence and strains 232, 7448, 7422, 168, NJ, and LH show high virulence, as determined through animal challenge experiments, complemented with in vitro tracheal mucosa infection tests. These 10 strains with known virulence were then subjected to classification via multilocus sequence typing (MLST) with three housekeeping genes, P146-based genotyping, and multilocus variable-number tandem-repeat analysis (MLVA) of 13 loci. MLST and P146-based genotyping identified 168, 168 L, NJ, and RM48 as the same type and clustered them in a single branch. MLVA assigned a different sequence type to each strain. Simpson's index of diversity indicates a higher discriminatory ability for MLVA. However, no statistically significant correlation was found between genotypes and virulence. Furthermore, we investigated the correlation between virulence and biofilm formation ability. The strains showing high virulence demonstrate strong biofilm formation ability, while attenuated strains show low biofilm formation ability. Pearson correlation analysis revealed a significant positive correlation between biofilm formation ability and virulence. To conclude, there was no association between virulence and our genotyping data, but virulence was found to be significantly associated with the biofilm formation ability of M. hyopneumoniae.

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