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Identification of dipeptidyl peptidase-IV inhibitory peptides from yak bone collagen by in silico and in vitro analysis

文献类型： 外文期刊

作者： Liu, Chunyu 1 ; Guo, Zitao 1 ; Yang, Yuliang 1 ; Hu, Bo 3 ; Zhu, Lingyu 1 ; Li, Moying 1 ; Gu, Zhenghua 1 ; Xin, Yu 1 ; Sun, Haiyan 4 ; Guan, Yanming 5 ; Zhang, Liang 1 ;

作者机构： 1.Jiangnan Univ, Natl Engn Res Ctr Cereal Fermentat & Food Biomfg, Wuxi 214122, Jiangsu, Peoples R China

2.Jiangnan Univ, Jiangsu Prov Res Ctr Bioact Prod Proc Technol, Wuxi 214122, Jiangsu, Peoples R China

3.Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan Key Lab Trop Microbe Resources, Haikou 571101, Hainan, Peoples R China

5.China Natl Res Inst Food & Fermentat Ind Co Ltd, Beijing 100015, Peoples R China

关键词： Yak bone collagen; In silico analysis; DPP-IV inhibitory; HepG2 cells; Insulin resistance

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：3.498； 五年影响因子：3.455 ）

ISSN： 1438-2377

年卷期： 2022 年 248 卷 12 期

页码：

收录情况： SCI

摘要： The fast development of bioinformatics and various established databases of bioactive peptides provide time-saving and efficient method for identifying bioactive peptides from various proteins. The yak bone collagen is rich in nutrition and have various potential bioactivities, but the study on yak bone collagen as a precursor of antidiabetic peptide has not been reported. In this study, yak bone collagen was first evaluated as a precursor to generate dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides by in silico analysis. After multi-cycles of screening, three peptides (GHR, GIR and MGPR) were screened out and synthesized to evaluate their inhibitory activities in vitro. Among the three peptides, MGPR had the best inhibitory effect on DPP-IV with IC50 of 0.490 +/- 0.012 mM. Further cell test verified that MGPR could ameliorate glucosamine-induced glucose uptake reduction in HepG2 cells. These results suggested that yak bone collagen could be applied as a good precursor for antidiabetic peptides. This study would further broaden the application of yak bone collagen in the fields of the food and pharmaceuticals industry. [GRAPHICS] .

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